LOCUS       sequence-149323-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress AKT1 in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Lee MS, Jeong MH, Lee HW, Han HJ, Ko A, Hewitt SM, Kim JH, Chun KH, 
            Chung JY, Lee C, Cho H, Song J
  TITLE     PI3K/AKT activation induces PTEN ubiquitination and destabilization 
            accelerating tumourigenesis.
  JOURNAL   Nat Commun. 2015 Jul 17;6:7769. doi: 10.1038/ncomms8769.
  PUBMED    26183061
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-17"; volume: "6:7769. doi"; pages: " 
            10.1038/ncomms8769"
COMMENT     Sequence Label: pcDNA3.1-HA AKT1
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             100..126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc atggcctcct acccttatga tgtgccagat
      121 tatgcctctc ccgaattcat gagcgacgtg gctattgtga aggagggttg gctgcacaaa
      181 cgaggggagt acatcaagac ctggcggcca cgctacttcc tcctcaagaa tgatggcacc
      241 ttcattggct acaaggagcg gccgcaggat gtggaccaac gtgaggctcc cctcaacaac
      301 ttctctgtgg cgcagtgcca gctgatgaag acggagcggc cccggcccaa caccttcatc
      361 atccgctgcc tgcagtggac cactgtcatc gaacgcacct tccatgtgga gactcctgag
      421 gagcgggagg agtggacaac cgccatccag actgtggctg acggcctcaa gaagcaggag
      481 gaggaggaga tggacttccg gtcgggctca cccagtgaca actcaggggc tgaagagatg
      541 gaggtgtccc tggccaagcc caagcaccgc gtgaccatga acgagtttga gtacctgaag
      601 ctgctgggca agggcacttt cggcaaggtg atcctggtga aggagaaggc cacaggccgc
      661 tactacgcca tgaagatcct caagaaggaa gtcatcgtag ccaaggacga agtggcccac
      721 acactcaccg agaaccgcgt cctgcagaac tccaggcacc ccttcctcac agccctgaag
      781 tactctttcc agacccacga ccgcctctgc tttgtcatgg agtacgccaa cgggggcgag
      841 ctgttcttcc acc
//
