LOCUS       sequence-149331-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress PTEN in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Lee MS, Jeong MH, Lee HW, Han HJ, Ko A, Hewitt SM, Kim JH, Chun KH, 
            Chung JY, Lee C, Cho H, Song J
  TITLE     PI3K/AKT activation induces PTEN ubiquitination and destabilization 
            accelerating tumourigenesis.
  JOURNAL   Nat Commun. 2015 Jul 17;6:7769. doi: 10.1038/ncomms8769.
  PUBMED    26183061
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-17"; volume: "6:7769. doi"; pages: " 
            10.1038/ncomms8769"
COMMENT     Sequence Label: pcDNA3-HA PTEN
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             98..124
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc ttggtaccat ggcctcctac ccttatgatg tgccagatta
      121 tgcctctccc gaattcatga cagccatcat caaagagatc gttagcagaa acaaaaggag
      181 atatcaagag gatggattcg acttagactt gacctatatt tatccaaaca ttattgctat
      241 gggatttcct gcagaaagac ttgaaggcgt atacaggaac aatattgatg atgtagtaag
      301 gtttttggat tcaaagcata aaaaccatta caagatatac aatctttgtg ctgaaagaca
      361 ttatgacacc gccaaattta attgcagagt tgcacaatat ccttttgaag accataaccc
      421 accacagcta gaacttatca aacccttttg tgaagatctt gaccaatggc taagtgaaga
      481 tgacaatcat gttgcagcaa ttcactgtaa agctggaaag ggacgaactg gtgtaatgat
      541 atgtgcatat ttattacatc ggggcaaatt tttaaaggca caagaggccc tagatttcta
      601 tggggaagta aggaccagag acaaaaaggg agtaactatt cccagtcaga ggcgctatgt
      661 gtattattat agctacctgt taaagaatca tctggattat agaccagtgg cactgttgtt
      721 tcacaagatg atgtttgaaa ctattccaat gttcagtggc ggaacttgca atcctcagtt
      781 t
//
