LOCUS       sequence-149334-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress  p53 2KR in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Lee EW, Lee MS, Camus S, Ghim J, Yang MR, Oh W, Ha NC, Lane DP, Song
            J
  TITLE     Differential regulation of p53 and p21 by MKRN1 E3 ligase controls 
            cell cycle arrest and apoptosis.
  JOURNAL   EMBO J. 2009 Jul 22;28(14):2100-13. doi: 10.1038/emboj.2009.164. 
            Epub 2009 Jun 18.
  PUBMED    19536131
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2009.164"; journalName: "EMBO J"; date: "2009-07-22- 
            22"; volume: "28"; issue: "14"; pages: "2100-13"
COMMENT     Sequence Label: pcDNA3-HA-p53 2KR
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             93..119
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttggt accatggcct cctaccctta tgatgtgcca gattatgcct
      121 ctcccgaatt catggaggag ccgcagtcag atcctagcgt cgagccccct ctgagtcagg
      181 aaacattttc agacctatgg aaactacttc ctgaaaacaa cgttctgtcc cccttgccgt
      241 cccaagcaat ggatgatttg atgctgtccc cggacgatat tgaacaatgg ttcactgaag
      301 acccaggtcc agatgaagct cccagaatgc cagaggctgc tccccccgtg gcccctgcac
      361 cagcagctcc tacaccggcg gcccctgcac cagccccctc ctggcccctg tcatcttctg
      421 tcccttccca gaaaacctac cagggcagct acggtttccg tctgggcttc ttgcattctg
      481 ggacagccaa gtctgtgact tgcacgtact cccctgccct caacaagatg ttttgccaac
      541 tggccaagac ctgccctgtg cagctgtggg ttgattccac acccccgccc ggcacccgcg
      601 tccgcgccat ggccatctac aagcagtcac agcacatgac ggaggttgtg aggcgctgcc
      661 cccaccatga gcgctgctca gatagcgatg gtctggcccc tcctcagcat cttatccgag
      721 tggaaggaaa tttgcgtgtg gagtatttgg atgacagaaa cacttttcga catagtgtgg
      781 tggtgcccta tgagccg
//
