LOCUS       sequence-149338-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress PTEN in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Lee MS, Jeong MH, Lee HW, Han HJ, Ko A, Hewitt SM, Kim JH, Chun KH, 
            Chung JY, Lee C, Cho H, Song J
  TITLE     PI3K/AKT activation induces PTEN ubiquitination and destabilization 
            accelerating tumourigenesis.
  JOURNAL   Nat Commun. 2015 Jul 17;6:7769. doi: 10.1038/ncomms8769.
  PUBMED    26183061
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-17"; volume: "6:7769. doi"; pages: " 
            10.1038/ncomms8769"
COMMENT     Sequence Label: pcDNA3-FLAG PTEN
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..121
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttgccgc caccatggat tacaaggatg acgacgataa
      121 gagcgcgggc ggatccgaat tcatgacagc catcatcaaa gagatcgtta gcagaaacaa
      181 aaggagatat caagaggatg gattcgactt agacttgacc tatatttatc caaacattat
      241 tgctatggga tttcctgcag aaagacttga aggcgtatac aggaacaata ttgatgatgt
      301 agtaaggttt ttggattcaa agcataaaaa ccattacaag atatacaatc tttgtgctga
      361 aagacattat gacaccgcca aatttaattg cagagttgca caatatcctt ttgaagacca
      421 taacccacca cagctagaac ttatcaaacc cttttgtgaa gatcttgacc aatggctaag
      481 tgaagatgac aatcatgttg cagcaattca ctgtaaagct ggaaagggac gaactggtgt
      541 aatgatatgt gcatatttat tacatcgggg caaattttta aaggcacaag aggccctaga
      601 tttctatggg gaagtaagga ccagagacaa aaagggagta actattccca gtcagaggcg
      661 ctatgtgtat tattatagct acctgttaaa gaatcatctg gattatagac cagtggcact
      721 gttgtttcac aagatgatgt ttgaaactat tccaatgttc agtggcgg
//
