LOCUS       sequence-149347-         572 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human codon optimized plasmid to express arC9 t2a mCherry w/2xNLS 
            and a sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Oakes BL, Nadler DC, Flamholz A, Fellmann C, Staahl BT, Doudna JA, 
            Savage DF
  TITLE     Profiling of engineering hotspots identifies an allosteric 
            CRISPR-Cas9 switch.
  JOURNAL   Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528.
  PUBMED    27136077
REFERENCE   2  (bases 1 to 572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528."
COMMENT     Sequence Label: pBLO1808_arC9_2xNLS_human
FEATURES             Location/Qualifiers
     source          1..572
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             44..109
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             116..136
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctgcctgaat cacttttttt caggttggac cggtgccacc atggactata aggaccacga
       61 cggagactac aaggatcatg atattgatta caaagacgat gacgataaga tggccccaaa
      121 gaagaagcgg aaggtcggta tccacggagt cccagcagcc gacaagaagt acagcatcgg
      181 cctggacatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc
      241 cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat
      301 cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga agagaaccgc
      361 cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa
      421 cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct tcctggtgga
      481 agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg aggtggccta
      541 ccacgagaag taccccacca tctaccacct ga
//
