LOCUS       sequence-149351-         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3d-Myc13
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..542
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
     terminator      605..792
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(660..684)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gtcgacggat ccccgggtta attaacgcta gcgcgattaa cggtgaacaa aagctaatct
       61 ccgaggaaga cttgaacggt gaacaaaaat taatctcaga agaagacttg aacggactcg
      121 acggtgaaca aaagttgatt tctgaagaag atttgaacgg tgaacaaaag ctaatctccg
      181 aggaagactt gaacggtgaa caaaaattaa tctcagaaga agacttgaac ggactcgacg
      241 gtgaacaaaa gttgatttct gaagaagatt tgaacggtga acaaaagcta atctccgagg
      301 aagacttgaa cggtgaacaa aaattaatct cagaagaaga cttgaacgga ctcgacggtg
      361 aacaaaagtt gatttctgaa gaagatttga acggtgaaca aaagctaatc tccgaggaag
      421 acttgaacgg tgaacaaaaa ttaatctcag aagaagactt gaacggactc gacggtgaac
      481 aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa gctaatctcc gaggaagact
      541 tgaacggtga acaaaaatta atcaatcagt acaagtctag atgaggcgcg ccacttctaa
      601 ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag
      661 tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa
      721 ctctttcctg taggtcaggt tgctttctca ggtatagtat gaggtcgctc ttattgacca
      781 cacctctacc ggcagatccg ctaggagatc tgagctcgat atcaagctta gctacaaatc
      841 ccactggcta tatgtatgca tttgtgttaa aaaagtttgt atagattatt taatctactc
      901 agcattcttt ctctaaatag ga
//
