LOCUS       sequence-149352-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3d-HA3
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..62
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             66..92
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             99..125
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      158..345
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(213..237)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gtcgacggat ccccgggtta attaacgcta gcatgtaccc atacgatgtt cctgactatg
       61 cgggctaccc gtatgacgtg ccggactatg caggagccta tccttatgac gttccagatt
      121 acgctggttc tagatgaggc gcgccacttc taaataagcg aatttcttat gatttatgat
      181 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta
      241 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc
      301 tcaggtatag tatgaggtcg ctcttattga ccacacctct accggcagat ccgctaggag
      361 atctgagctc gatatcaagc ttagctacaa atcccactgg ctatatgtat gcatttgtgt
      421 taaaaaagtt tgtatagatt atttaatcta ctcagcattc tttctctaaa taggaatttg
      481 ttacttaatg gagaaaaaaa tgtttcgatt tacctagtgt atttgtttgt atactcacgt
      541 ttaatttcaa acatccattc tatcttgtgt aatttttggc atggtgaaaa agataatcag
      601 ccttataatc tttacaaaag taagaaattc tgtaaataag ccttaatgcc cttgctttaa
      661 attaaaatgg ttctttttca tgataatgtt tgcactttgt gaatatattt tagatagttc
      721 tgtgaggtat aattaagatg ttttagagac ttatacaatt ttgtctttat aaattcttaa
      781 ttgattttac catcccagtt taactatgct tcgtcggcat ctctgcacat gtcgtgtttt
      841 cttaccgtat tgtcctacca agaacctctt ttttgcttgg atcgaaatta aaggtttaaa
      901 agcaaagtta tggatgctag agtatttc
//
