LOCUS       sequence-149356-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3d-FL3
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..71
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             75..122
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     terminator      152..339
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(207..231)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 acgctgcagg tcgacggatc cccgggttaa ttaacgctag catgggtgac tacaaggacg
       61 acgatgacaa gggagattac aaagatgacg acgataagga ctacaaggac gacgatgaca
      121 agtctagatg aggcgcgcca cttctaaata agcgaatttc ttatgattta tgatttttat
      181 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt
      241 aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt
      301 atagtatgag gtcgctctta ttgaccacac ctctaccggc agatccgcta ggagatctga
      361 gctcgatatc aagcttagct acaaatccca ctggctatat gtatgcattt gtgttaaaaa
      421 agtttgtata gattatttaa tctactcagc attctttctc taaataggaa tttgttactt
      481 aatggagaaa aaaatgtttc gatttaccta gtgtatttgt ttgtatactc acgtttaatt
      541 tcaaacatcc attctatctt gtgtaatttt tggcatggtg aaaaagataa tcagccttat
      601 aatctttaca aaagtaagaa attctgtaaa taagccttaa tgcccttgct ttaaattaaa
      661 atggttcttt ttcatgataa tgtttgcact ttgtgaatat attttagata gttctgtgag
      721 gtataattaa gatgttttag agacttatac aattttgtct ttataaattc ttaattgatt
      781 ttaccatccc agtttaacta tgcttcgtcg gcatctctgc acatgtcgtg ttttcttacc
      841 gtattgtcct accaagaacc tcttttttgc ttggatcgaa attaaaggtt taaaagcaaa
      901 gttatggatg ctagagtatt tcaaagctat tcagctagag ctgaggggat gaaaaatccc
      961 attgccaagg aattgttggc tttgatgg
//
