LOCUS       sequence-149363-         249 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Spinach2 under a T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Wang XC, Wilson SC, Hammond MC
  TITLE     Next-generation RNA-based fluorescent biosensors enable anaerobic 
            detection of cyclic di-GMP.
  JOURNAL   Nucleic Acids Res. 2016 Jul 5. pii: gkw580.
  PUBMED    27382070
REFERENCE   2  (bases 1 to 249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jul 5. pii: gkw580."
COMMENT     Sequence Label: pET31b-T7-Spinach2
FEATURES             Location/Qualifiers
     source          1..249
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..34
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15..33
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      203..249
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cgatcccgcg aaattaatac gactcactat aggggcccgg atagctcagt cggtagagca
       61 gcggccggat gtaactgaat gaaatggtga aggacgggtc cagtaggctg cttcggcagc
      121 ctacttgttg agtagagtgt gagctccgta actagttaca tccggccgcg ggtccagggt
      181 tcaagtccct gttcgggcgc catagcataa ccccttgggg cctctaaacg ggtcttgagg
      241 ggttttttg
//
