LOCUS       sequence-149364-         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3d-PK3
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..83
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             87..128
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             129..170
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     terminator      200..387
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(255..279)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 acgctgcagg tcgacggatc cccgggttaa ttaacgctag cggtaagcct attcctaacc
       61 ctcttttggg tctcgattct acaggtggaa aaccaatccc caacccactc ctcggccttg
      121 actcaactgg caaacccata ccaaatcctt tacttggatt agatagtacc tctagatgag
      181 gcgcgccact tctaaataag cgaatttctt atgatttatg atttttatta ttaaataagt
      241 tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt
      301 cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat agtatgaggt
      361 cgctcttatt gaccacacct ctaccggcag atccgctagg agatctgagc tcgatatcaa
      421 gcttagctac aaatcccact ggctatatgt atgcatttgt gttaaaaaag tttgtataga
      481 ttatttaatc tactcagcat tctttctcta aataggaatt tgttacttaa tggagaaaaa
      541 aatgtttcga tttacctagt gtatttgttt gtatactcac gtttaatttc aaacatccat
      601 tctatcttgt gtaatttttg gcatggtgaa aaagataatc agccttataa tctttacaaa
      661 agtaagaaat tctgtaaata agccttaatg cccttgcttt aaattaaaat ggttcttttt
      721 catgataatg tttgcacttt gtgaatatat tttagatagt tctgtgaggt ataattaaga
      781 tgttttagag acttatacaa ttttgtcttt ataaattctt aattgatttt accatcccag
      841 tttaactatg cttcgtcggc atctctgcac atgtcgtgtt ttcttaccgt attgtcctac
      901 caagaacctc ttttttgctt ggatcgaaat taaaggttta aaagcaaagt tatggatgct
      961 agagtatttc
//
