LOCUS       sequence-149367-        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3c-PK9
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..84
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             88..129
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             130..171
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             178..219
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             223..264
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             265..306
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             313..354
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             358..399
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             400..441
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     terminator      474..661
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(529..553)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 tacgctgcag gtcgacggat ccccgggtta attaacgcta gcggtaagcc tattcctaac
       61 cctcttttgg gtctcgattc tacaggtgga aaaccaatcc ccaacccact cctcggcctt
      121 gactcaactg gcaaacccat accaaatcct ttacttggat tagatagtac ctctagcggt
      181 aagcctattc ctaaccctct tttgggtctc gattctacag gtggaaaacc aatccccaac
      241 ccactcctcg gccttgactc aactggcaaa cccataccaa atcctttact tggattagat
      301 agtacctcta gcggtaagcc tattcctaac cctcttttgg gtctcgattc tacaggtgga
      361 aaaccaatcc ccaacccact cctcggcctt gactcaactg gcaaacccat accaaatcct
      421 ttacttggat tagatagtac ctctagagat tgaggcgcgc cacttctaaa taagcgaatt
      481 tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat
      541 tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt
      601 aggtcaggtt gctttctcag gtatagtatg aggtcgctct tattgaccac acctctaccg
      661 gcagatccgc taggagatct gtttagcttg cctcgtcccc gccgggtcac ccggccagcg
      721 acatggaggc ccagaatacc ctccttgaca gtcttgacgt gcgcagctca ggggcatgat
      781 gtgactgtcg cccgtatatt tagcccacac atccccatgt ataatcattt gcatccatac
      841 attttgatgg ctgcacggcg cgaagcaaaa attacggctc ctcgctgcag acctgcgagc
      901 agggaaacgc tcccctcaca gacgcgttga attgtcccca cgccgcgccc ctgtagagaa
      961 atataaaagg ttaggatttg ccactgaggt tcttctttca tatacttcct tttaaaatct
     1021 tgctaggata ca
//
