LOCUS       sequence-149387-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian DamID vector for fusing gene of interest with Dam-V5 using
            Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Vogel MJ, Guelen L, de Wit E, Peric-Hupkes D, Loden M, Talhout W, 
            Feenstra M, Abbas B, Classen AK, van Steensel B
  TITLE     Human heterochromatin proteins form large domains containing 
            KRAB-ZNF genes.
  JOURNAL   Genome Res. 2006 Dec;16(12):1493-504. Epub 2006 Oct 12.
  PUBMED    17038565
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2006-12"; volume: "16(12)"; pages: "1493-504. Epub 
            2006 Oct 12"
COMMENT     Sequence Label: pINDgw RFA-V5-EcoDam
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    148..272
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(297..302,303..320,321..327)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(447..466)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 acaagttacc gagaaagaag aactcacaca cagctagcgt ttaaacttaa gcttggtacc
       61 gagctcggat ccactagtcc agtgtggtgg aattctgcag atatccagca cagtggcggc
      121 cgctcgagtc tagagggccc ttcgatcaca agtttgtaca aaaaagctga acgagaaacg
      181 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
      241 tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta
      301 cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt
      361 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
      421 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
      481 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
      541 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc
      601 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
      661 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
      721 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaa
//
