LOCUS       sequence-149388-        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which 
            contains eSpCas9 and puromycin cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Sikder RK, Ellithi M, Uzzo RN, Weader D, Metz AL, Behbahani A, 
            McKenzie ER, El-Deiry WS, Abbosh PH
  TITLE     Differential effects of clinically-relevant N- vs C-terminal 
            truncating CDKN1A mutations on cisplatin sensitivity in bladder 
            cancer.
  JOURNAL   Mol Cancer Res. 2020 Dec 3. pii: 1541-7786.MCR-19-1200. doi: 
            10.1158/1541-7786.MCR-19-1200.
  PUBMED    33272936
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2020 Dec 3. pii: 1541-7786.MCR-19-1200. doi: 
            10.1158/1541-7786.MCR-19-1200."
COMMENT     Sequence Label: pLentiCRISPR-E
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg
       61 agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac
      121 accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg
      181 ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg
      241 accatatcgt gcctcagagc tttctggccg acgactccat cgacaacaag gtgctgacca
      301 gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga
      361 tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca
      421 atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga
      481 gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga
      541 tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga
      601 agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca
      661 acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca
      721 aaaagtaccc tgcgctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc
      781 ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct
      841 acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga
      901 aggcgcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg
      961 attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg
     1021 aggtgca
//
