LOCUS       sequence-149389-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which 
            contains eSpCas9 and puromycin cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Sikder RK, Ellithi M, Uzzo RN, Weader D, Metz AL, Behbahani A, 
            McKenzie ER, El-Deiry WS, Abbosh PH
  TITLE     Differential effects of clinically-relevant N- vs C-terminal 
            truncating CDKN1A mutations on cisplatin sensitivity in bladder 
            cancer.
  JOURNAL   Mol Cancer Res. 2020 Dec 3. pii: 1541-7786.MCR-19-1200. doi: 
            10.1158/1541-7786.MCR-19-1200.
  PUBMED    33272936
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2020 Dec 3. pii: 1541-7786.MCR-19-1200. doi: 
            10.1158/1541-7786.MCR-19-1200."
COMMENT     Sequence Label: pLentiCRISPR-E
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
       61 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
      121 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
      181 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctgc gctggaaagc
      241 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
      301 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
      361 aagaccgaga ttaccctggc caacggcgag atccggaagg cgcctctgat cgagacaaac
      421 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
      481 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
      541 aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
      601 gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg
      661 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
      721 accatcatgg aaagaagcag cttc
//
