LOCUS       sequence-149393-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express mCherry-GFP1-10 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Lee JG, Takahama S, Zhang G, Tomarev SI, Ye Y
  TITLE     Unconventional secretion of misfolded proteins promotes adaptation 
            to proteasome dysfunction in mammalian cells.
  JOURNAL   Nat Cell Biol. 2016 Jul;18(7):765-76. doi: 10.1038/ncb3372. Epub 
            2016 Jun 13.
  PUBMED    27295555
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3372"; 
            journalName: "Nat Cell Biol"; date: "2016-07"; volume: "18"; issue: 
            "7"; pages: "765-76"
COMMENT     Sequence Label: mCherry-GFP1-10
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             27..734
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(172..190)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat aacatggcca
       61 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
      121 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
      181 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
      241 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
      301 tccccgaggg cttcaattgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
      361 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
      421 ccaacttccc ctccgacggc cccgtaatgc agtgtcgtac catgggctgg gaggcctcca
      481 ctgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
      541 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
      601 tgcagctgcc cggcgcctac aacgtcgaca tcaagttgga catcctttcc cacaacgagg
      661 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
      721 acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgaattcc atggtttcga
      781 aaggcgagga gctgttcaca ggcgtggtgc caatcctggt ggagctggac ggcgacgtga
//
