LOCUS       sequence-149402-       10198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insulin receptor isoform A with a inter-domain TagBFP tag at 
            extracellular region (between furin-like domain and transmembrane 
            domain). For imaging insulin receptor trafficing..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10198)
  AUTHORS   Boothe T, Lim GE, Cen H, Skovso S, Piske M, Li SN, Nabi IR, Gilon P,
            Johnson JD
  TITLE     Inter-domain tagging implicates caveolin-1 in insulin receptor 
            trafficking and Erk signaling bias in pancreatic beta-cells.
  JOURNAL   Mol Metab. 2016 Feb 10;5(5):366-78. doi: 
            10.1016/j.molmet.2016.01.009. eCollection 2016 May.
  PUBMED    27110488
REFERENCE   2  (bases 1 to 10198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molmet.2016.01.009"; journalName: "Mol Metab"; date: 
            "2016-02-10- 10"; volume: "5"; issue: "5"; pages: "366-78"
COMMENT     Sequence Label: InsRA-TagBFP
FEATURES             Location/Qualifiers
     source          1..10198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      915..924
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3753..4448
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     primer_bind     complement(5792..5809)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5798..6022
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6068..6496
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6155..6174)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6365..6386
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6505..6525)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6510..6839
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6690..6825
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6906..7700
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6960..6979)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7570..7589
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7874..7995
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7911..7930)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7965..7984
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8044..8060)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8044..8060)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8057..8079)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8068..8084
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8092..8098,8099..8116,8117..8122))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8137..8158
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8275..8292)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8446..9031)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8526..8545)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9202..9993,9994..10062))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9825..9844
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10063..10167)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gcccgccgcc atgggcaccg ggggccggcg gggggcggcg gccgcgccgc
      961 tgctggtggc ggtggccgcg ctgctactgg gcgccgcggg ccacctgtac cccggagagg
     1021 tgtgtcccgg catggatatc cggaacaacc tcactaggtt gcatgagctg gagaattgct
     1081 ctgtcatcga aggacacttg cagatactct tgatgttcaa aacgaggccc gaagatttcc
     1141 gagacctcag tttccccaaa ctcatcatga tcactgatta cttgctgctc ttccgggtct
     1201 atgggctcga gagcctgaag gacctgttcc ccaacctcac ggtcatccgg ggatcacgac
     1261 tgttctttaa ctacgcgctg gtcatcttcg agatggttca cctcaaggaa ctcggcctct
     1321 acaacctgat gaacatcacc cggggttctg tccgcatcga gaagaacaat gagctctgtt
     1381 acttggccac tatcgactgg tcccgtatcc tggattccgt ggaggataat cacatcgtgt
     1441 tgaacaaaga tgacaacgag gagtgtggag acatctgtcc gggtaccgcg aagggcaaga
     1501 ccaactgccc cgccaccgtc atcaacgggc agtttgtcga acgatgttgg actcatagtc
     1561 actgccagaa agtttgcccg accatctgta agtcacacgg ctgcaccgcc gaaggcctct
     1621 gttgccacag cgagtgcctg ggcaactgtt ctcagcccga cgaccccacc aagtgcgtgg
     1681 cctgccgcaa cttctacctg gacggcaggt gtgtggagac ctgcccgccc ccgtactacc
     1741 acttccagga ctggcgctgt gtgaacttca gcttctgcca ggacctgcac cacaaatgca
     1801 agaactcgcg gaggcagggc tgccaccaat acgtcattca caacaacaag tgcatccctg
     1861 agtgtccctc cgggtacacg atgaattcca gcaacttgct gtgcacccca tgcctgggtc
     1921 cctgtcccaa ggtgtgccac ctcctagaag gcgagaagac catcgactcg gtgacgtctg
     1981 cccaggagct ccgaggatgc accgtcatca acgggagtct gatcatcaac attcgaggag
     2041 gcaacaatct ggcagctgag ctagaagcca acctcggcct cattgaagaa atttcagggt
     2101 atctaaaaat ccgccgatcc tacgctctgg tgtcactttc cttcttccgg aagttacgtc
     2161 tgattcgagg agagaccttg gaaattggga actactcctt ctatgccttg gacaaccaga
     2221 acctaaggca gctctgggac tggagcaaac acaacctcac caccactcag gggaaactct
     2281 tcttccacta taaccccaaa ctctgcttgt cagaaatcca caagatggaa gaagtttcag
     2341 gaaccaaggg gcgccaggag agaaacgaca ttgccctgaa gaccaatggg gacaaggcat
     2401 cctgtgaaaa tgagttactt aaattttctt acattcggac atcttttgac aagatcttgc
     2461 tgagatggga gccgtactgg ccccccgact tccgagacct cttggggttc atgctgttct
     2521 acaaagaggc cccttatcag aatgtgacgg agttcgatgg gcaggatgcg tgtggttcca
     2581 acagttggac ggtggtagac attgacccac ccctgaggtc caacgacccc aaatcacaga
     2641 accacccagg gtggctgatg cggggtctca agccctggac ccagtatgcc atctttgtga
     2701 agaccctggt caccttttcg gatgaacgcc ggacctatgg ggccaagagt gacatcattt
     2761 atgtccagac agatgccacc aacccctctg tgcccctgga tccaatctca gtgtctaact
     2821 catcatccca gattattctg aagtggaaac caccctccga ccccaatggc aacatcaccc
     2881 actacctggt tttctgggag aggcaggcgg aagacagtga gctgttcgag ctggattatt
     2941 gcctcaaagg gctgaagctg ccctcgagga cctggtctcc accattcgag tctgaagatt
     3001 ctcagaagca caaccagagt gagtatgagg attcggccgg cgaatgctgc tcctgtccaa
     3061 agacagactc tcagatcctg aaggagctgg aggagtcctc gtttaggaag acgtttgagg
     3121 attacctgca caacgtggtt ttcgtcccca ggccatctcg gaaacgcagg tcccttggcg
     3181 atgttgggaa tgtgacggtg gccgtgccca cggtggcagc tttccccaac acttcctcga
     3241 ccagcgtgcc cacgagtccg gaggagcaca ggccttttga gaaggtggtg aacaaggagt
     3301 cgctggtcat ctccggcttg cgacacttca cgggctatcg catcgagctg caggcttgca
     3361 accaggacac ccctgaggaa cggtgcagtg tggcagccta cgtcagtgcg aggaccatgc
     3421 ctgaagccaa ggctgatgac attgttggcc ctgtgacgca tgaaatcttt gagaacaacg
     3481 tcgtccactt gatgtggcag gagccgaagg agcccaatgg tctgatcgtg ctgtatgaag
     3541 tgagttatcg gcgatatggt gatgaggagc tgcatctctg cgtctcccgc aagcacttcg
     3601 ctctggaacg gggctgcagg ctgcgtgggc tgtcaccggg gaactacagc gtgcgaatcc
     3661 gggccacctc ccttgcgggc aacggctctt ggacggaacc cacctatttc tacgtgacag
     3721 actatttaga cgtccgtacg atcgaattcc ccagcgagct gattaaggag aacatgcaca
     3781 tgaagctgta catggagggc accgtggaca accatcactt caagtgcaca tccgagggcg
     3841 aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc
     3901 tccccttcgc cttcgacatc ctggctacta gcttcctcta cggcagcaag accttcatca
     3961 accacaccca gggcatcccc gacttcttca agcagtcctt ccctgagggc ttcacatggg
     4021 agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc
     4081 aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttcaca tccaacggcc
     4141 ctgtgatgca gaagaaaaca ctcggctggg aggccttcac cgagacgctg taccccgctg
     4201 acggcggcct ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg agccatctga
     4261 tcgcaaacat caagaccaca tatagatcca agaaacccgc taagaacctc aagatgcctg
     4321 gcgtctacta tgtggactac agactggaaa gaatcaagga ggccaacaac gagacctacg
     4381 tcgagcagca cgaggtggca gtggccagat actgcgacct ccctagcaaa ctggggcaca
     4441 agcttaattc cggatcagat accggtccgt caaatattgc aaaaattatc atcggccccc
     4501 tcatctttgt ctttctcttc agtgttgtga ttggaagtat ttatctattc ctgagaaaga
     4561 ggcagccaga tgggccgctg ggaccgcttt acgcttcttc aaaccctgag tatctcagtg
     4621 ccagtgatgt gtttccatgc tctgtgtacg tgccggacga gtgggaggtg tctcgagaga
     4681 agatcaccct ccttcgagag ctggggcagg gctccttcgg catggtgtat gagggcaatg
     4741 ccagggacat catcaagggt gaggcagaga cccgcgtggc ggtgaagacg gtcaacgagt
     4801 cagccagtct ccgagagcgg attgagttcc tcaatgaggc ctcggtcatg aagggcttca
     4861 cctgccatca cgtggtgcgc ctcctgggag tggtgtccaa gggccagccc acgctggtgg
     4921 tgatggagct gatggctcac ggagacctga agagctacct ccgttctctg cggccagagg
     4981 ctgagaataa tcctggccgc cctcccccta cccttcaaga gatgattcag atggcggcag
     5041 agattgctga cgggatggcc tacctgaacg ccaagaagtt tgtgcatcgg gacctggcag
     5101 cgagaaactg catggtcgcc catgatttta ctgtcaaaat tggagacttt ggaatgacca
     5161 gagacatcta tgaaacggat tactaccgga aagggggcaa gggtctgctc cctgtacggt
     5221 ggatggcacc ggagtccctg aaggatgggg tcttcaccac ttcttctgac atgtggtcct
     5281 ttggcgtggt cctttgggaa atcaccagct tggcagaaca gccttaccaa ggcctgtcta
     5341 atgaacaggt gttgaaattt gtcatggatg gagggtatct ggatcaaccc gacaactgtc
     5401 cagagagagt cactgacctc atgcgcatgt gctggcaatt caaccccaag atgaggccaa
     5461 ccttcctgga gattgtcaac ctgctcaagg acgacctgca ccccagcttt ccagaggtgt
     5521 cgttcttcca cagcgaggag aacaaggctc ccgagagtga ggagctggag atggagtttg
     5581 aggacatgga gaatgtgccc ctggaccgtt cctcgcactg tcagagggag gaggcggggg
     5641 gccgggatgg agggtcctcg ctgggtttca agcggagcta cgaggaacac atcccttaca
     5701 cacacatgaa cggaggcaag aaaaacgggc ggattctgac cttgcctcgg tccaatcctt
     5761 cctaaaagct taagtttaaa ccgctgatca gcctcgactg tgccttctag ttgccagcca
     5821 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     5881 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     5941 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     6001 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     6061 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     6121 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     6181 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     6241 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     6301 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     6361 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     6421 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6481 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     6541 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     6601 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6661 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     6721 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     6781 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     6841 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     6901 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     6961 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     7021 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     7081 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     7141 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     7201 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     7261 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     7321 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     7381 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     7441 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     7501 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     7561 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     7621 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     7681 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     7741 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     7801 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     7861 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7921 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7981 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     8041 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     8101 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     8161 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8221 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8281 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8341 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8401 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8461 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8521 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8581 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8641 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8701 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8761 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8821 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8881 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8941 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtttttttg tttgcaagca
     9001 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9061 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9121 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9181 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9241 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9301 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9361 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9421 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9481 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9541 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9601 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9661 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9721 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9781 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9841 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9901 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9961 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10021 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10081 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10141 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
