LOCUS       sequence-149406-       10216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insulin receptor isoform A with a inter-domain eGFP tag at 
            extracellular region (between furin-like domain and transmembrane 
            domain). For imaging insulin receptor trafficing..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Boothe T, Lim GE, Cen H, Skovso S, Piske M, Li SN, Nabi IR, Gilon P,
            Johnson JD
  TITLE     Inter-domain tagging implicates caveolin-1 in insulin receptor 
            trafficking and Erk signaling bias in pancreatic beta-cells.
  JOURNAL   Mol Metab. 2016 Feb 10;5(5):366-78. doi: 
            10.1016/j.molmet.2016.01.009. eCollection 2016 May.
  PUBMED    27110488
REFERENCE   2  (bases 1 to 10216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molmet.2016.01.009"; journalName: "Mol Metab"; date: 
            "2016-02-10- 10"; volume: "5"; issue: "5"; pages: "366-78"
COMMENT     Sequence Label: InsRA-eGFP
FEATURES             Location/Qualifiers
     source          1..10216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      915..924
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3753..3755,3756..4466)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3753..3755,3756..4466)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3795..3816)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4056..4075)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4403..4424
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5810..5827)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5816..6040
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6086..6514
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6173..6192)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6383..6404
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6523..6543)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6528..6857
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6708..6843
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6924..7718
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6978..6997)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7588..7607
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7892..8013
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7929..7948)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7983..8002
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8062..8078)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8062..8078)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8075..8097)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8086..8102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8110..8116,8117..8134,8135..8140))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8155..8176
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8293..8310)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8464..9049)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8544..8563)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9220..10011,10012..10080))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9843..9862
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10081..10185)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gcccgccgcc atgggcaccg ggggccggcg gggggcggcg gccgcgccgc
      961 tgctggtggc ggtggccgcg ctgctactgg gcgccgcggg ccacctgtac cccggagagg
     1021 tgtgtcccgg catggatatc cggaacaacc tcactaggtt gcatgagctg gagaattgct
     1081 ctgtcatcga aggacacttg cagatactct tgatgttcaa aacgaggccc gaagatttcc
     1141 gagacctcag tttccccaaa ctcatcatga tcactgatta cttgctgctc ttccgggtct
     1201 atgggctcga gagcctgaag gacctgttcc ccaacctcac ggtcatccgg ggatcacgac
     1261 tgttctttaa ctacgcgctg gtcatcttcg agatggttca cctcaaggaa ctcggcctct
     1321 acaacctgat gaacatcacc cggggttctg tccgcatcga gaagaacaat gagctctgtt
     1381 acttggccac tatcgactgg tcccgtatcc tggattccgt ggaggataat cacatcgtgt
     1441 tgaacaaaga tgacaacgag gagtgtggag acatctgtcc gggtaccgcg aagggcaaga
     1501 ccaactgccc cgccaccgtc atcaacgggc agtttgtcga acgatgttgg actcatagtc
     1561 actgccagaa agtttgcccg accatctgta agtcacacgg ctgcaccgcc gaaggcctct
     1621 gttgccacag cgagtgcctg ggcaactgtt ctcagcccga cgaccccacc aagtgcgtgg
     1681 cctgccgcaa cttctacctg gacggcaggt gtgtggagac ctgcccgccc ccgtactacc
     1741 acttccagga ctggcgctgt gtgaacttca gcttctgcca ggacctgcac cacaaatgca
     1801 agaactcgcg gaggcagggc tgccaccaat acgtcattca caacaacaag tgcatccctg
     1861 agtgtccctc cgggtacacg atgaattcca gcaacttgct gtgcacccca tgcctgggtc
     1921 cctgtcccaa ggtgtgccac ctcctagaag gcgagaagac catcgactcg gtgacgtctg
     1981 cccaggagct ccgaggatgc accgtcatca acgggagtct gatcatcaac attcgaggag
     2041 gcaacaatct ggcagctgag ctagaagcca acctcggcct cattgaagaa atttcagggt
     2101 atctaaaaat ccgccgatcc tacgctctgg tgtcactttc cttcttccgg aagttacgtc
     2161 tgattcgagg agagaccttg gaaattggga actactcctt ctatgccttg gacaaccaga
     2221 acctaaggca gctctgggac tggagcaaac acaacctcac caccactcag gggaaactct
     2281 tcttccacta taaccccaaa ctctgcttgt cagaaatcca caagatggaa gaagtttcag
     2341 gaaccaaggg gcgccaggag agaaacgaca ttgccctgaa gaccaatggg gacaaggcat
     2401 cctgtgaaaa tgagttactt aaattttctt acattcggac atcttttgac aagatcttgc
     2461 tgagatggga gccgtactgg ccccccgact tccgagacct cttggggttc atgctgttct
     2521 acaaagaggc cccttatcag aatgtgacgg agttcgatgg gcaggatgcg tgtggttcca
     2581 acagttggac ggtggtagac attgacccac ccctgaggtc caacgacccc aaatcacaga
     2641 accacccagg gtggctgatg cggggtctca agccctggac ccagtatgcc atctttgtga
     2701 agaccctggt caccttttcg gatgaacgcc ggacctatgg ggccaagagt gacatcattt
     2761 atgtccagac agatgccacc aacccctctg tgcccctgga tccaatctca gtgtctaact
     2821 catcatccca gattattctg aagtggaaac caccctccga ccccaatggc aacatcaccc
     2881 actacctggt tttctgggag aggcaggcgg aagacagtga gctgttcgag ctggattatt
     2941 gcctcaaagg gctgaagctg ccctcgagga cctggtctcc accattcgag tctgaagatt
     3001 ctcagaagca caaccagagt gagtatgagg attcggccgg cgaatgctgc tcctgtccaa
     3061 agacagactc tcagatcctg aaggagctgg aggagtcctc gtttaggaag acgtttgagg
     3121 attacctgca caacgtggtt ttcgtcccca ggccatctcg gaaacgcagg tcccttggcg
     3181 atgttgggaa tgtgacggtg gccgtgccca cggtggcagc tttccccaac acttcctcga
     3241 ccagcgtgcc cacgagtccg gaggagcaca ggccttttga gaaggtggtg aacaaggagt
     3301 cgctggtcat ctccggcttg cgacacttca cgggctatcg catcgagctg caggcttgca
     3361 accaggacac ccctgaggaa cggtgcagtg tggcagccta cgtcagtgcg aggaccatgc
     3421 ctgaagccaa ggctgatgac attgttggcc ctgtgacgca tgaaatcttt gagaacaacg
     3481 tcgtccactt gatgtggcag gagccgaagg agcccaatgg tctgatcgtg ctgtatgaag
     3541 tgagttatcg gcgatatggt gatgaggagc tgcatctctg cgtctcccgc aagcacttcg
     3601 ctctggaacg gggctgcagg ctgcgtgggc tgtcaccggg gaactacagc gtgcgaatcc
     3661 gggccacctc ccttgcgggc aacggctctt ggacggaacc cacctatttc tacgtgacag
     3721 actatttaga cgtccgtacg atcgaattcc ccgtgagcaa gggcgaggag ctgttcaccg
     3781 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     3841 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     3901 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     3961 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     4021 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     4081 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     4141 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     4201 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     4261 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     4321 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     4381 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     4441 ctctcggcat ggacgagctg tacaagtccg gatcagatac cggtccgtca aatattgcaa
     4501 aaattatcat cggccccctc atctttgtct ttctcttcag tgttgtgatt ggaagtattt
     4561 atctattcct gagaaagagg cagccagatg ggccgctggg accgctttac gcttcttcaa
     4621 accctgagta tctcagtgcc agtgatgtgt ttccatgctc tgtgtacgtg ccggacgagt
     4681 gggaggtgtc tcgagagaag atcaccctcc ttcgagagct ggggcagggc tccttcggca
     4741 tggtgtatga gggcaatgcc agggacatca tcaagggtga ggcagagacc cgcgtggcgg
     4801 tgaagacggt caacgagtca gccagtctcc gagagcggat tgagttcctc aatgaggcct
     4861 cggtcatgaa gggcttcacc tgccatcacg tggtgcgcct cctgggagtg gtgtccaagg
     4921 gccagcccac gctggtggtg atggagctga tggctcacgg agacctgaag agctacctcc
     4981 gttctctgcg gccagaggct gagaataatc ctggccgccc tccccctacc cttcaagaga
     5041 tgattcagat ggcggcagag attgctgacg ggatggccta cctgaacgcc aagaagtttg
     5101 tgcatcggga cctggcagcg agaaactgca tggtcgccca tgattttact gtcaaaattg
     5161 gagactttgg aatgaccaga gacatctatg aaacggatta ctaccggaaa gggggcaagg
     5221 gtctgctccc tgtacggtgg atggcaccgg agtccctgaa ggatggggtc ttcaccactt
     5281 cttctgacat gtggtccttt ggcgtggtcc tttgggaaat caccagcttg gcagaacagc
     5341 cttaccaagg cctgtctaat gaacaggtgt tgaaatttgt catggatgga gggtatctgg
     5401 atcaacccga caactgtcca gagagagtca ctgacctcat gcgcatgtgc tggcaattca
     5461 accccaagat gaggccaacc ttcctggaga ttgtcaacct gctcaaggac gacctgcacc
     5521 ccagctttcc agaggtgtcg ttcttccaca gcgaggagaa caaggctccc gagagtgagg
     5581 agctggagat ggagtttgag gacatggaga atgtgcccct ggaccgttcc tcgcactgtc
     5641 agagggagga ggcggggggc cgggatggag ggtcctcgct gggtttcaag cggagctacg
     5701 aggaacacat cccttacaca cacatgaacg gaggcaagaa aaacgggcgg attctgacct
     5761 tgcctcggtc caatccttcc taaaagctta agtttaaacc gctgatcagc ctcgactgtg
     5821 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     5881 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     5941 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     6001 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     6061 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     6121 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     6181 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     6241 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     6301 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     6361 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6421 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6481 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     6541 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6601 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6661 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     6721 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6781 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6841 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
     6901 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     6961 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     7021 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     7081 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     7141 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     7201 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     7261 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     7321 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     7381 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     7441 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     7501 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     7561 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     7621 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     7681 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     7741 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     7801 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     7861 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     7921 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7981 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     8041 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8101 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8161 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8221 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8281 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8341 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8401 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8461 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8521 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8581 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8641 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8701 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8761 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8821 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8881 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8941 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9001 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9061 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9121 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     9181 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9241 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9301 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9361 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9421 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9481 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9541 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9601 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9661 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9721 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9781 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9841 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9901 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9961 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10021 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10081 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10141 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    10201 agtgccacct gacgtc
//
