LOCUS       sequence-149419-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress p14ARF in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Ko A, Shin JY, Seo J, Lee KD, Lee EW, Lee MS, Lee HW, Choi IJ, Jeong
            JS, Chun KH, Song J
  TITLE     Acceleration of gastric tumorigenesis through MKRN1-mediated 
            posttranslational regulation of p14ARF.
  JOURNAL   J Natl Cancer Inst. 2012 Nov 7;104(21):1660-72. doi: 
            10.1093/jnci/djs424. Epub 2012 Oct 25.
  PUBMED    23104211
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/jnci/djs424"; journalName: "J Natl Cancer Inst"; date: 
            "2012-11-7- 7"; volume: "104"; issue: "21"; pages: "1660-72"
COMMENT     Sequence Label: pCS5-3HA/p14ARF
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        53..71
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     53..70
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             534..560
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             540..566
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             573..599
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             579..605
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             609..635
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             615..641
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     primer_bind     complement(659..678)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(660..678)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(707..726)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     761..780
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 catgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct tgatttaggt
       61 gacactatag aatacaagct acttgttctt tttgcaggat ccaaatggtg cgcaggttct
      121 tggtgaccct ccggattcgg cgcgcgtgcg gcccgccgcg agtgagggtt ttcgtggttc
      181 acatcccgcg gctcacgggg gagtgggcag cgccaggggc gcccgccgct gtggccctcg
      241 tgctgatgct actgaggagc cagcgtctag ggcagcagcc gcttcctaga agaccaggtc
      301 atgatgatgg gcagcgcccg agtggcggag ctgctgctgc tccacggcgc ggagcccaac
      361 tgcgccgacc ccgccactct cacccgaccc gtgcacgacg ctgcccggga gggcttcctg
      421 gacacgctgg tggtgctgca ccgggccggg gcgcggctgg acgtgcgcga tgcctggggc
      481 cgtctgcccg tggacctggc ttgatatctg gcgcgcctac catggggccc ggctacccct
      541 acgatgtgcc tgactacgct tccctgggcg gctaccccta cgatgtgcct gactacgctt
      601 ccctgggcta cccctacgat gtgcctgact acgcttccct gggctagata gctagcctcc
      661 ctatagtgag tcgtattacg tagatccaga catgataaga tacattgatg agtttggaca
      721 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
      781 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
      841 tatgtttcag gttcaggggg aggtgtggga ggttttttaa ttcgcggccg cggcgcccaa
      901 tgcattgggc ccggtaccca gcttttgttc cctttagtga gggttaaatt gcgcgc
//
