LOCUS       sequence-149420-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress p14ARF in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Ko A, Shin JY, Seo J, Lee KD, Lee EW, Lee MS, Lee HW, Choi IJ, Jeong
            JS, Chun KH, Song J
  TITLE     Acceleration of gastric tumorigenesis through MKRN1-mediated 
            posttranslational regulation of p14ARF.
  JOURNAL   J Natl Cancer Inst. 2012 Nov 7;104(21):1660-72. doi: 
            10.1093/jnci/djs424. Epub 2012 Oct 25.
  PUBMED    23104211
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/jnci/djs424"; journalName: "J Natl Cancer Inst"; date: 
            "2012-11-7- 7"; volume: "104"; issue: "21"; pages: "1660-72"
COMMENT     Sequence Label: pCS5-3HA/p14ARF
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..57)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(37..63)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(67..93)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(73..99)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(106..132)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(112..138)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(601..619)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(602..619)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 actcactata gggaggctag ctatctagcc cagggaagcg tagtcaggca catcgtaggg
       61 gtagcccagg gaagcgtagt caggcacatc gtaggggtag ccgcccaggg aagcgtagtc
      121 aggcacatcg taggggtagc cgggccccat ggtaggcgcg ccagatatca agccaggtcc
      181 acgggcagac ggccccaggc atcgcgcacg tccagccgcg ccccggcccg gtgcagcacc
      241 accagcgtgt ccaggaagcc ctcccgggca gcgtcgtgca cgggtcgggt gagagtggcg
      301 gggtcggcgc agttgggctc cgcgccgtgg agcagcagca gctccgccac tcgggcgctg
      361 cccatcatca tgacctggtc ttctaggaag cggctgctgc cctagacgct ggctcctcag
      421 tagcatcagc acgagggcca cagcggcggg cgcccctggc gctgcccact cccccgtgag
      481 ccgcgggatg tgaaccacga aaaccctcac tcgcggcggg ccgcacgcgc gccgaatccg
      541 gagggtcacc aagaacctgc gcaccatttg gatcctgcaa aaagaacaag tagcttgtat
      601 tctatagtgt cacctaaatc aagcttgctc cgacgtcccc aggcagaatg gcggttccct
      661 aaacgagcat tgcttatata gacctcccat taggcacgcc taccgcccat ttacgtcaat
      721 ggaacgccca tttgcgtcat tgcccctccc cattgacgtc aatggggatg tacttggcag
      781 ccatcgcggg ccatttaccg ccattgacgt caatgggagt actgccaatg taccctggcg
      841 tacttcaata gtaatgtact tgccaagtta
//
