LOCUS       sequence-149421-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress ARF65-132 in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Ko A, Shin JY, Seo J, Lee KD, Lee EW, Lee MS, Lee HW, Choi IJ, Jeong
            JS, Chun KH, Song J
  TITLE     Acceleration of gastric tumorigenesis through MKRN1-mediated 
            posttranslational regulation of p14ARF.
  JOURNAL   J Natl Cancer Inst. 2012 Nov 7;104(21):1660-72. doi: 
            10.1093/jnci/djs424. Epub 2012 Oct 25.
  PUBMED    23104211
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/jnci/djs424"; journalName: "J Natl Cancer Inst"; date: 
            "2012-11-7- 7"; volume: "104"; issue: "21"; pages: "1660-72"
COMMENT     Sequence Label: pCS3-MT-BX/ARF65-132
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        46..64
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     46..63
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             114..143
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             153..182
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             192..221
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             231..260
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             270..299
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             333..362
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    668..802
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(692..711)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     746..765
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cgtttaggga ccgccattct gcctggggac gtcggagcaa gcttgattta ggtgacacta
       61 tagaatacaa gctacttgtt ctttttgcag gatcccatcg atttaaagct atggagcaaa
      121 agctcatttc tgaagaggac ttgaatgaaa tggagcaaaa gctcatttct gaagaggact
      181 tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg gagcaaaagc
      241 tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa gaggacttga
      301 atgaaatgga gagcttgggc gacctcacca tggagcaaaa gctcatttct gaagaggact
      361 tgaatttaga gatctcgagc tcaagcttcg aattcatggg tcatgatgat gggcagcgcc
      421 cgagtggcgg agctgctgct gctccacggc gcggagccca actgcgccga ccccgccact
      481 ctcacccgac ccgtgcacga cgctgcccgg gagggcttcc tggacacgct ggtggtgctg
      541 caccgggccg gggcgcggct ggacgtgcgc gatgcctggg gccgtctgcc cgtggacctg
      601 gctaactcga cggtaccgcg ggcccgggat ccaccggatc tagaactata gtgagtcgta
      661 ttacgtagat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
      721 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
      781 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
//
