LOCUS       sequence-149422-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress ARF65-132 in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Ko A, Shin JY, Seo J, Lee KD, Lee EW, Lee MS, Lee HW, Choi IJ, Jeong
            JS, Chun KH, Song J
  TITLE     Acceleration of gastric tumorigenesis through MKRN1-mediated 
            posttranslational regulation of p14ARF.
  JOURNAL   J Natl Cancer Inst. 2012 Nov 7;104(21):1660-72. doi: 
            10.1093/jnci/djs424. Epub 2012 Oct 25.
  PUBMED    23104211
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/jnci/djs424"; journalName: "J Natl Cancer Inst"; date: 
            "2012-11-7- 7"; volume: "104"; issue: "21"; pages: "1660-72"
COMMENT     Sequence Label: pCS3-MT-BX/ARF65-132
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(296..325)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(359..388)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(398..427)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(437..466)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(476..505)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(515..544)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(594..612)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(595..612)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 actcactata gtttctagat ccggtggatc ccgggcccgc ggtaccgtcg agttagccag
       61 gtccacgggc agacggcccc aggcatcgcg cacgtccagc cgcgccccgg cccggtgcag
      121 caccaccagc gtgtccagga agccctcccg ggcagcgtcg tgcacgggtc gggtgagagt
      181 ggcggggtcg gcgcagttgg gctccgcgcc gtggagcagc agcagctccg ccactcgggc
      241 gctgcccatc atcatgaccc atgaattcga agcttgagct cgagatctct aaattcaagt
      301 cctcttcaga aatgagcttt tgctccatgg tgaggtcgcc caagctctcc atttcattca
      361 agtcctcttc agaaatgagc ttttgctcca tttcattcaa gtcctcttca gaaatgagct
      421 tttgctccat ttcattcaag tcctcttcag aaatgagctt ttgctccatt tcattcaagt
      481 cctcttcaga aatgagcttt tgctccattt cattcaagtc ctcttcagaa atgagctttt
      541 gctccatagc tttaaatcga tgggatcctg caaaaagaac aagtagcttg tattctatag
      601 tgtcacctaa atcaagcttg ctccgacgtc cccaggcaga atggcggttc cctaaacgag
      661 cattgcttat atagacctcc cattaggcac gcctaccgcc catttacgtc aatggaacgc
      721 ccatttgcgt
//
