LOCUS       sequence-149424-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress ARF1-64 in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Ko A, Shin JY, Seo J, Lee KD, Lee EW, Lee MS, Lee HW, Choi IJ, Jeong
            JS, Chun KH, Song J
  TITLE     Acceleration of gastric tumorigenesis through MKRN1-mediated 
            posttranslational regulation of p14ARF.
  JOURNAL   J Natl Cancer Inst. 2012 Nov 7;104(21):1660-72. doi: 
            10.1093/jnci/djs424. Epub 2012 Oct 25.
  PUBMED    23104211
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/jnci/djs424"; journalName: "J Natl Cancer Inst"; date: 
            "2012-11-7- 7"; volume: "104"; issue: "21"; pages: "1660-72"
COMMENT     Sequence Label: pCS3-MT-BX/ARF1-64
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(281..310)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(344..373)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(383..412)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(422..451)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(461..490)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(500..529)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(579..597)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(580..597)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gactcactat agttctagat ccggtggatc ccgggcccgc ggtaccgtcg agttatggtc
       61 ttctaggaag cggctgctgc cctagacgct ggctcctcag tagcatcagc acgagggcca
      121 cagcggcggg cgcccctggc gctgcccact cccccgtgag ccgcgggatg tgaaccacga
      181 aaaccctcac tcgcggcggg ccgcacgcgc gccgaatccg gagggtcacc aagaacctgc
      241 gcaccatgaa ttcgaagctt gagctcgaga tctctaaatt caagtcctct tcagaaatga
      301 gcttttgctc catggtgagg tcgcccaagc tctccatttc attcaagtcc tcttcagaaa
      361 tgagcttttg ctccatttca ttcaagtcct cttcagaaat gagcttttgc tccatttcat
      421 tcaagtcctc ttcagaaatg agcttttgct ccatttcatt caagtcctct tcagaaatga
      481 gcttttgctc catttcattc aagtcctctt cagaaatgag cttttgctcc atagctttaa
      541 atcgatggga tcctgcaaaa agaacaagta gcttgtattc tatagtgtca cctaaatcaa
      601 gcttgctccg acgtccccag gcagaatggc ggttccctaa acgagcattg cttatataga
      661 cctcccatta ggcacgccta ccgcccattt acgtcaatgg aacgcccatt tgcgtcattg
      721 cccctcccca ttgacgtcaa tgggggatgt acttggcagc catcgcgggc catttaccgc
      781 cattgacgtc aatgggagta
//
