LOCUS       sequence-149425-        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG 
            promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Saarimaki-Vire J, Balboa D, Russell MA, Saarikettu J, Kinnunen M, 
            Keskitalo S, Malhi A, Valensisi C, Andrus C, Eurola S, Grym H, 
            Ustinov J, Wartiovaara K, Hawkins RD, Silvennoinen O, Varjosalo M, 
            Morgan NG, Otonkoski T
  TITLE     An Activating STAT3 Mutation Causes Neonatal Diabetes through 
            Premature Induction of Pancreatic Differentiation.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):281-294. doi: 
            10.1016/j.celrep.2017.03.055.
  PUBMED    28402852
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "281-294"
COMMENT     Sequence Label: CAG-Cas9-T2A-EGFP-ires-puro
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(282..335)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(360..380)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg
       61 aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg
      121 ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
      181 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
      241 accccggtga acagctcctc gcccttgctc accatatcga ttgggccagg attctcctcg
      301 acgtcaccgc atgttagcag acttcctctg ccctctccgg agccgtcgag gctggcctcc
      361 acctttctct tcttcttggg gctgtcgcct cccagctgag acaggtcgat ccgtgtctcg
      421 tacaggccgg tgatgctctg gtggatcagg gtggcgtcca gcacctcttt ggtgctggtg
      481 tacctcttcc ggtcgatggt ggtgtcaaag tacttgaagg cggcaggggc tcccagattg
      541 gtcagggtaa acaggtggat gatattctcg gcctgctctc tgatgggctt atcccggtgc
      601 ttgttgtagg cggacagcac tttgtccaga ttagcgtcgg ccaggatcac tctcttggag
      661 aactcgctga tctgctcgat gatctcgtcc aggtagtgct tgtgctgttc cacaaacagc
      721 tgtttctgct cattatcctc gggggagccc ttcagcttct catagtggct ggccaggtac
      781 aggaagttca catatttgga gggcagggcc agttcgtttc ccttctgcag ttcgccggca
      841 gaggccagca ttctcttccg gccgttttcc agctcgaaca gggagtactt aggcagcttg
      901 atgatcaggt cctttttcac ttctttgtag cccttggctt ccagaaagtc gatgggattc
      961 ttctcgaagc tgcttctttc catgatggtg atccccagca gctcttt
//
