LOCUS       sequence-149426-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG 
            promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Saarimaki-Vire J, Balboa D, Russell MA, Saarikettu J, Kinnunen M, 
            Keskitalo S, Malhi A, Valensisi C, Andrus C, Eurola S, Grym H, 
            Ustinov J, Wartiovaara K, Hawkins RD, Silvennoinen O, Varjosalo M, 
            Morgan NG, Otonkoski T
  TITLE     An Activating STAT3 Mutation Causes Neonatal Diabetes through 
            Premature Induction of Pancreatic Differentiation.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):281-294. doi: 
            10.1016/j.celrep.2017.03.055.
  PUBMED    28402852
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "281-294"
COMMENT     Sequence Label: CAG-Cas9-T2A-EGFP-ires-puro
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(46..645)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(129..149)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     626..645
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(819..838)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 tcgtgcgctc ctttcggtcg ggcgctgcgg gtcgtggggc gggcgtcagg caccgggctt
       61 gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa
      121 gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac
      181 cccggcgcgc tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc
      241 ctggtggtcg ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg
      301 cggcgccagg aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc
      361 catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca
      421 gaccgccacc gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg
      481 cgtgaggaag agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt
      541 gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac
      601 gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg gtcatggttg tggccatatt
      661 atcatcgtgt ttttcaaagg aaaaccacgt ccccgtggtt cggggggcct agacgttttt
      721 ttaacctcga ctaaacacat gtaaagcatg tgcaccgagg ccccagatca gatcccatac
      781 aatggggtac cttctgggca tccttcagcc ccttgttgaa tacgcttgag gagagccatt
      841 tgactctttc cacaactatc caactcacaa cgtggcactg gggttgtgcc gcctttgcag
      901 gtgtatctta tacacgtggc ttttggcc
//
