LOCUS       sequence-149427-         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress p14ARF in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Ko A, Shin JY, Seo J, Lee KD, Lee EW, Lee MS, Lee HW, Choi IJ, Jeong
            JS, Chun KH, Song J
  TITLE     Acceleration of gastric tumorigenesis through MKRN1-mediated 
            posttranslational regulation of p14ARF.
  JOURNAL   J Natl Cancer Inst. 2012 Nov 7;104(21):1660-72. doi: 
            10.1093/jnci/djs424. Epub 2012 Oct 25.
  PUBMED    23104211
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/jnci/djs424"; journalName: "J Natl Cancer Inst"; date: 
            "2012-11-7- 7"; volume: "104"; issue: "21"; pages: "1660-72"
COMMENT     Sequence Label: pCS3-MT-BX/p14ARF
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        49..67
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     49..66
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             117..146
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             156..185
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             195..224
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             234..263
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             273..302
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             336..365
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(884..903)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     938..957
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctcgtttagg gaaccgccat tctgcctggg gacgtcggag caagcttgat ttaggtgaca
       61 ctatagaata caagctactt gttctttttg caggatccca tcgatttaaa gctatggagc
      121 aaaagctcat ttctgaagag gacttgaatg aaatggagca aaagctcatt tctgaagagg
      181 acttgaatga aatggagcaa aagctcattt ctgaagagga cttgaatgaa atggagcaaa
      241 agctcatttc tgaagaggac ttgaatgaaa tggagcaaaa gctcatttct gaagaggact
      301 tgaatgaaat ggagagcttg ggcgacctca ccatggagca aaagctcatt tctgaagagg
      361 acttgaattt agagatctcg agctcaagct tcgaattcat ggtgcgcagg ttcttggtga
      421 ccctccggat tcggcgcgcg tgcggcccgc cgcgagtgag ggttttcgtg gttcacatcc
      481 cgcggctcac gggggagtgg gcagcgccag gggcgcccgc cgctgtggcc ctcgtgctga
      541 tgctactgag gagccagcgt ctagggcagc agccgcttcc tagaagacca ggtcatgatg
      601 atgggcagcg cccgagtggc ggagctgctg ctgctccacg gcgcggagcc caactgcgcc
      661 gaccccgcca ctctcacccg acccgtgcac gacgctgccc gggagggctt cctggacacg
      721 ctggtggtgc tgcaccgggc cggggcgcgg ctggacgtgc gcgatgcctg gggccgtctg
      781 cccgtggacc tggctaactc gacggtaccg cgggcccggg atccaccgga tctagaacta
      841 tagtgagtcg tattacgtag atccagacat gataagatac attgatgagt ttggacaaac
      901 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
      961 atttgtaacc attataagct
//
