LOCUS       sequence-149430-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress p14ARF in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Ko A, Shin JY, Seo J, Lee KD, Lee EW, Lee MS, Lee HW, Choi IJ, Jeong
            JS, Chun KH, Song J
  TITLE     Acceleration of gastric tumorigenesis through MKRN1-mediated 
            posttranslational regulation of p14ARF.
  JOURNAL   J Natl Cancer Inst. 2012 Nov 7;104(21):1660-72. doi: 
            10.1093/jnci/djs424. Epub 2012 Oct 25.
  PUBMED    23104211
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/jnci/djs424"; journalName: "J Natl Cancer Inst"; date: 
            "2012-11-7- 7"; volume: "104"; issue: "21"; pages: "1660-72"
COMMENT     Sequence Label: pCS3-MT-BX/p14ARF
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(484..513)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(547..576)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(586..615)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(625..654)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(664..693)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(703..732)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(782..800)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(783..800)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 actcactata gttctagatc cggtggatcc cgggcccgcg gtaccgtcga gttagccagg
       61 tccacgggca gacggcccca ggcatcgcgc acgtccagcc gcgccccggc ccggtgcagc
      121 accaccagcg tgtccaggaa gccctcccgg gcagcgtcgt gcacgggtcg ggtgagagtg
      181 gcggggtcgg cgcagttggg ctccgcgccg tggagcagca gcagctccgc cactcgggcg
      241 ctgcccatca tcatgacctg gtcttctagg aagcggctgc tgccctagac gctggctcct
      301 cagtagcatc agcacgaggg ccacagcggc gggcgcccct ggcgctgccc actcccccgt
      361 gagccgcggg atgtgaacca cgaaaaccct cactcgcggc gggccgcacg cgcgccgaat
      421 ccggagggtc accaagaacc tgcgcaccat gaattcgaag cttgagctcg agatctctaa
      481 attcaagtcc tcttcagaaa tgagcttttg ctccatggtg aggtcgccca agctctccat
      541 ttcattcaag tcctcttcag aaatgagctt ttgctccatt tcattcaagt cctcttcaga
      601 aatgagcttt tgctccattt cattcaagtc ctcttcagaa atgagctttt gctccatttc
      661 attcaagtcc tcttcagaaa tgagcttttg ctccatttca ttcaagtcct cttcagaaat
      721 gagcttttgc tccatagctt taaatcgatg ggatcctgca aaaagaacaa gtagcttgta
      781 ttctatagtg tcacctaaat caagcttgct ccgacgtccc
//
