LOCUS       sequence-149432-         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress p14ARF in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Ko A, Shin JY, Seo J, Lee KD, Lee EW, Lee MS, Lee HW, Choi IJ, Jeong
            JS, Chun KH, Song J
  TITLE     Acceleration of gastric tumorigenesis through MKRN1-mediated 
            posttranslational regulation of p14ARF.
  JOURNAL   J Natl Cancer Inst. 2012 Nov 7;104(21):1660-72. doi: 
            10.1093/jnci/djs424. Epub 2012 Oct 25.
  PUBMED    23104211
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/jnci/djs424"; journalName: "J Natl Cancer Inst"; date: 
            "2012-11-7- 7"; volume: "104"; issue: "21"; pages: "1660-72"
COMMENT     Sequence Label: p3xFLAG-CMV14/p14ARF
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             489..554
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(764..783)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 cagagctcgt ttagtgaacc gtcagaatta agcttatggt gcgcaggttc ttggtgaccc
       61 tccggattcg gcgcgcgtgc ggcccgccgc gagtgagggt tttcgtggtt cacatcccgc
      121 ggctcacggg ggagtgggca gcgccagggg cgcccgccgc tgtggccctc gtgctgatgc
      181 tactgaggag ccagcgtcta gggcagcagc cgcttcctag aagaccaggt catgatgatg
      241 ggcagcgccc gagtggcgga gctgctgctg ctccacggcg cggagcccaa ctgcgccgac
      301 cccgccactc tcacccgacc cgtgcacgac gctgcccggg agggcttcct ggacacgctg
      361 gtggtgctgc accgggccgg ggcgcggctg gacgtgcgcg atgcctgggg ccgtctgccc
      421 gtggacctgg caagaattca tcgatagatc tgatatcggt accagtcgac tctagaggat
      481 cccgggctga ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg
      541 atgacgatga caagtagtga tcccgggtgg catccctgtg acccctcccc agtgcctctc
      601 ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc
      661 atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg
      721 agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc
      781 tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt
      841 ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa
      901 tttttgtttt tttggtagag acg
//
