LOCUS       sequence-149433-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress p14ARF in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Ko A, Shin JY, Seo J, Lee KD, Lee EW, Lee MS, Lee HW, Choi IJ, Jeong
            JS, Chun KH, Song J
  TITLE     Acceleration of gastric tumorigenesis through MKRN1-mediated 
            posttranslational regulation of p14ARF.
  JOURNAL   J Natl Cancer Inst. 2012 Nov 7;104(21):1660-72. doi: 
            10.1093/jnci/djs424. Epub 2012 Oct 25.
  PUBMED    23104211
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/jnci/djs424"; journalName: "J Natl Cancer Inst"; date: 
            "2012-11-7- 7"; volume: "104"; issue: "21"; pages: "1660-72"
COMMENT     Sequence Label: p3xFLAG-CMV14/p14ARF
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(182..247)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(729..828)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(758..778)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cacttgcccc ttgctccata ccacccccct ccaccccata atattataga aggacaccta
       61 gtcagacaaa atgatgcaac ttaattttat taggacaagg ctggtgggca ctggagtggc
      121 aacttccagg gccaggagag gcactgggga ggggtcacag ggatgccacc cgggatcact
      181 acttgtcatc gtcatccttg tagtcgatgt catgatcttt ataatcaccg tcatggtctt
      241 tgtagtcagc ccgggatcct ctagagtcga ctggtaccga tatcagatct atcgatgaat
      301 tcttgccagg tccacgggca gacggcccca ggcatcgcgc acgtccagcc gcgccccggc
      361 ccggtgcagc accaccagcg tgtccaggaa gccctcccgg gcagcgtcgt gcacgggtcg
      421 ggtgagagtg gcggggtcgg cgcagttggg ctccgcgccg tggagcagca gcagctccgc
      481 cactcgggcg ctgcccatca tcatgacctg gtcttctagg aagcggctgc tgccctagac
      541 gctggctcct cagtagcatc agcacgaggg ccacagcggc gggcgcccct ggcgctgccc
      601 actcccccgt gagccgcggg atgtgaacca cgaaaaccct cactcgcggc gggccgcacg
      661 cgcgccgaat ccggagggtc accaagaacc tgcgcaccat aagcttaatt ctgacggttc
      721 actaaacgag ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc
      781 aacggggcgg ggttattacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa
      841 ctcccattga cgtcaatggg ggtggagact tggaaatccc cgtgagtcaa
//
