LOCUS       sequence-149435-        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  psiCHECK2 dual luciferase reporter harboring a fully complementary 
            miR-34 target element, cloned into the XhoI/NotI restriction sites, 
            the 3'UTR of the Renilla Luciferase gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Salzman DW, Nakamura K, Nallur S, Dookwah MT, Metheetrairut C, Slack
            FJ, Weidhaas JB
  TITLE     miR-34 activity is modulated through 5'-end phosphorylation in 
            response to DNA damage.
  JOURNAL   Nat Commun. 2016 Mar 21;7:10954. doi: 10.1038/ncomms10954.
  PUBMED    26996824
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-21"; volume: "7:10954. doi"; pages: " 
            10.1038/ncomms10954"
COMMENT     Sequence Label: psiCHECK2-miR-34 WT
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             716..724
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 aagcggccgg ccgccccgac tctagaatta ttacacggcg atcttgccgc ctttcttagc
       61 cttgatcagg atctcgcgga tcttgcgggc gtccagcttg ccggtcaggc ccttgggcac
      121 ctcgtccacg aacaccactc cgccgcgcag cttcttggcg gttgtcacct ggctggccac
      181 atagtccacg atctccttct cggtcatggt cttgccgtgt tccagcacga cgacggcggc
      241 aggcagctcg ccggcatcgt cgtcgggcag gccggccact ccggcgtcga aaatgttagg
      301 gtgctgcagc aggatagact ccagctcggc tggggccacc tggtagccct tgtacttgat
      361 cagagacttc aggcggtcca cgatgaagaa gtgctcgtcc tcgtcccagt aggcaatgtc
      421 gccagagtgc agccagccgt ccttgtcgat cagggcgttt gtggcctcag ggttattcac
      481 gtagccggac ataatcatag ggccgcgcac acacagctcg ccgcgctggt tcactcccag
      541 ggtcttgccg gtgtccaggt ccaccacctt ggcctcgaag aatggcacca ccttgcccac
      601 ggcgccaggc ttgtcgtcgc cctctggggt aatcagaatg gcgctggttg tctcggtcag
      661 gccgtagccc tggcggatgc ctggcagatg aaagcgcttg gccacggctt cgcccacctc
      721 cttagacagt ggggcgccgc cagaggcaat ctcgtgcagg ttagacaggt cgtacttgtc
      781 gatcagggtg ctcttagcga agaagctgaa cagggttggc accagcaggg cagactgaat
      841 tttgtagtct tgcaggctgc gcaggaacag ctcctcctcg aagcggtaca tcagcaccac
      901 ccgaaagccg caaatcaggt agcccagggt ggtgaacatg ccgaagccgt ggtggaatgg
      961 caccacgctc agaatagcgg tgtcggggat gatctggttg cc
//
