LOCUS       sequence-149442-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast drug selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pFA6a-natCX
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             70..642
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      648..845
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttaca tgggtaccac tcttgacgac acggcttacc ggtaccgcac cagtgtcccg
      121 ggggacgccg aggccatcga ggcactggat gggtccttca ccaccgacac cgtcttccgc
      181 gtcaccgcca ccggggacgg cttcaccctg cgggaggtgc cggtggaccc gcccctgacc
      241 aaggtgttcc ccgacgacga atcggacgac gaatcggacg acggggagga cggcgacccg
      301 gactcccgga cgttcgtcgc gtacggggac gacggcgacc tggcgggctt cgtggtcgtc
      361 tcgtactccg gctggaaccg ccggctgacc gtcgaggaca tcgaggtcgc cccggagcac
      421 cgggggcacg gggtcgggcg cgcgttgatg gggctcgcga cggagttcgc ccgcgagcgg
      481 ggcgccgggc acctctggct ggaggtcacc aacgtcaacg caccggcgat ccacgcgtac
      541 cggcggatgg ggttcaccct ctgcggcctg gacaccgccc tgtacgacgg caccgcctcg
      601 gacggcgagc aggcgctcta catgagcatg ccctgcccct aatcagtact gacaataaaa
      661 agattcttgt tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat
      721 tttaatcaaa tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg
      781 cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta
      841 tactgctgtc gattc
//
