LOCUS       sequence-149445-         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast drug selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pFA6a-natCX
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(168..365)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ttggccgatt cattaatgca ggttaacctg gcttatcgaa attaatacga ctcactatag
       61 ggagaccggc agatccgcgg ccgcataggc cactagtgga tctgatatca tcgatgaatt
      121 cgagctcgtt taaacactgg atggcggcgt tagtatcgaa tcgacagcag tatagcgacc
      181 agcattcaca tacgattgac gcatgatatt actttctgcg cacttaactt cgcatctggg
      241 cagatgatgt cgaggcgaaa aaaaatataa atcacgctaa catttgatta aaatagaaca
      301 actacaatat aaaaaaacta tacaaatgac aagttcttga aaacaagaat ctttttattg
      361 tcagtactga ttaggggcag ggcatgctca tgtagagcgc ctgctcgccg tccgaggcgg
      421 tgccgtcgta cagggcggtg tccaggccgc agagggtgaa ccccatccgc cggtacgcgt
      481 ggatcgccgg tgcgttgacg ttggtgacct ccagccagag gtgcccggcg ccccgctcgc
      541 gggcgaactc cgtcgcgagc cccatcaacg cgcgcccgac cccgtgcccc cggtgctccg
      601 gggcgacctc gatgtcctcg acggtcagcc ggcggttcca gccggagtac gagacgacca
      661 cgaagcccgc caggtcgccg tcgtc
//
