LOCUS       sequence-149449-        1094 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  psiCHECK2 dual luciferase reporter harboring a fully complementary 
            miR-34 target element, cloned into the XhoI/NotI restriction sites, 
            the 3'UTR of the Renilla Luciferase gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Salzman DW, Nakamura K, Nallur S, Dookwah MT, Metheetrairut C, Slack
            FJ, Weidhaas JB
  TITLE     miR-34 activity is modulated through 5'-end phosphorylation in 
            response to DNA damage.
  JOURNAL   Nat Commun. 2016 Mar 21;7:10954. doi: 10.1038/ncomms10954.
  PUBMED    26996824
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-21"; volume: "7:10954. doi"; pages: " 
            10.1038/ncomms10954"
COMMENT     Sequence Label: psiCHECK2-miR-34 WT
FEATURES             Location/Qualifiers
     source          1..1094
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    243..291
                     /note="synthetic polyadenylation signal"
     promoter        300..1051
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      1081..1090
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cctaagatgt tcatcgagtc cgaccctggg ttcttttcca acgctattgt cgagggagct
       61 aagaagttcc ctaacaccga gttcgtgaag gtgaagggcc tccacttcag ccaggaggac
      121 gctccagatg aaatgggtaa gtacatcaag agcttcgtgg agcgcgtgct gaagaacgag
      181 cagtaattct aggcgatcgc tcgagtcaac cagctaagac actgccagcg gccgctggcc
      241 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaggatctaa
      301 atgagtcttc ggacctcgcg ggggccgctt aagcggtggt tagggtttgt ctgacgcggg
      361 gggaggggga aggaacgaaa cactctcatt cggaggcggc tcggggtttg gtcttggtgg
      421 ccacgggcac gcagaagagc gccgcgatcc tcttaagcac ccccccgccc tccgtggagg
      481 cgggggtttg gtcggcgggt ggtaactggc gggccgctga ctcgggcggg tcgcgcgccc
      541 cagagtgtga ccttttcggt ctgctcgcag acccccgggc ggcgccgccg cggcggcgac
      601 gggctcgctg ggtcctaggc tccatgggga ccgtatacgt ggacaggctc tggagcatcc
      661 gcacgactgc ggtgatatta ccggagacct tctgcgggac gagccgggtc acgcggctga
      721 cgcggagcgt ccgttgggcg acaaacacca ggacggggca caggtacact atcttgtcac
      781 ccggaggcgc gagggactgc aggagcttca gggagtggcg cagctgcttc atccccgtgg
      841 cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat atatttgcat gtctttagtt
      901 ctatgatgac acaaaccccg cccagcgtct tgtcattggc gaattcgaac acgcagatgc
      961 agtcggggcg gcgcggtccc aggtccactt cgcatattaa ggtgacgcgt gtggcctcga
     1021 acaccgagcg accctgcagc gacccgctta aaagcttggc attccggtac tgttggtaaa
     1081 gccaccatgg ccga
//
