LOCUS       sequence-149451-        1093 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  psiCHECK2 dual luciferase reporter harboring a fully complementary 
            let-7 target element, cloned into the XhoI/NotI restriction sites, 
            the 3'UTR of the Renilla Luciferase gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Salzman DW, Nakamura K, Nallur S, Dookwah MT, Metheetrairut C, Slack
            FJ, Weidhaas JB
  TITLE     miR-34 activity is modulated through 5'-end phosphorylation in 
            response to DNA damage.
  JOURNAL   Nat Commun. 2016 Mar 21;7:10954. doi: 10.1038/ncomms10954.
  PUBMED    26996824
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-21"; volume: "7:10954. doi"; pages: " 
            10.1038/ncomms10954"
COMMENT     Sequence Label: psiCHECK2-let-7 WT
FEATURES             Location/Qualifiers
     source          1..1093
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    237..285
                     /note="synthetic polyadenylation signal"
     promoter        294..1045
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      1075..1084
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgttcatcg agtccgaccc tgggttcttt tccaacgcta ttgtcgaggg agctaagaag
       61 ttccctaaca ccgagttcgt gaaggtgaag ggcctccact tcagccagga ggacgctcca
      121 gatgaaatgg gtaagtacat caagagcttc gtggagcgcg tgctgaagaa cgagcagtaa
      181 ttctaggcga tcgctcgaga actatacaac ctactacctc agcggccgct ggccgcaata
      241 aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaggat ctaaatgagt
      301 cttcggacct cgcgggggcc gcttaagcgg tggttagggt ttgtctgacg cggggggagg
      361 gggaaggaac gaaacactct cattcggagg cggctcgggg tttggtcttg gtggccacgg
      421 gcacgcagaa gagcgccgcg atcctcttaa gcaccccccc gccctccgtg gaggcggggg
      481 tttggtcggc gggtggtaac tggcgggccg ctgactcggg cgggtcgcgc gccccagagt
      541 gtgacctttt cggtctgctc gcagaccccc gggcggcgcc gccgcggcgg cgacgggctc
      601 gctgggtcct aggctccatg gggaccgtat acgtggacag gctctggagc atccgcacga
      661 ctgcggtgat attaccggag accttctgcg ggacgagccg ggtcacgcgg ctgacgcgga
      721 gcgtccgttg ggcgacaaac accaggacgg ggcacaggta cactatcttg tcacccggag
      781 gcgcgaggga ctgcaggagc ttcagggagt ggcgcagctg cttcatcccc gtggcccgtt
      841 gctcgcgttt gctggcggtg tccccggaag aaatatattt gcatgtcttt agttctatga
      901 tgacacaaac cccgcccagc gtcttgtcat tggcgaattc gaacacgcag atgcagtcgg
      961 ggcggcgcgg tcccaggtcc acttcgcata ttaaggtgac gcgtgtggcc tcgaacaccg
     1021 agcgaccctg cagcgacccg cttaaaagct tggcattccg gtactgttgg taaagccacc
     1081 atggccgatg cta
//
