LOCUS       sequence-149452-        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  psiCHECK2 dual luciferase reporter harboring a fully complementary 
            let-7 target element, cloned into the XhoI/NotI restriction sites, 
            the 3'UTR of the Renilla Luciferase gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Salzman DW, Nakamura K, Nallur S, Dookwah MT, Metheetrairut C, Slack
            FJ, Weidhaas JB
  TITLE     miR-34 activity is modulated through 5'-end phosphorylation in 
            response to DNA damage.
  JOURNAL   Nat Commun. 2016 Mar 21;7:10954. doi: 10.1038/ncomms10954.
  PUBMED    26996824
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-21"; volume: "7:10954. doi"; pages: " 
            10.1038/ncomms10954"
COMMENT     Sequence Label: psiCHECK2-let-7 WT
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             712..720
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggccggccgc cccgactcta gaattattac acggcgatct tgccgccttt cttagccttg
       61 atcaggatct cgcggatctt gcgggcgtcc agcttgccgg tcaggccctt gggcacctcg
      121 tccacgaaca ccactccgcc gcgcagcttc ttggcggttg tcacctggct ggccacatag
      181 tccacgatct ccttctcggt catggtcttg ccgtgttcca gcacgacgac ggcggcaggc
      241 agctcgccgg catcgtcgtc gggcaggccg gccactccgg cgtcgaaaat gttagggtgc
      301 tgcagcagga tagactccag ctcggctggg gccacctggt agcccttgta cttgatcaga
      361 gacttcaggc ggtccacgat gaagaagtgc tcgtcctcgt cccagtaggc aatgtcgcca
      421 gagtgcagcc agccgtcctt gtcgatcagg gcgtttgtgg cctcagggtt attcacgtag
      481 ccggacataa tcatagggcc gcgcacacac agctcgccgc gctggttcac tcccagggtc
      541 ttgccggtgt ccaggtccac caccttggcc tcgaagaatg gcaccacctt gcccacggcg
      601 ccaggcttgt cgtcgccctc tggggtaatc agaatggcgc tggttgtctc ggtcaggccg
      661 tagccctggc ggatgcctgg cagatgaaag cgcttggcca cggcttcgcc cacctcctta
      721 gacagtgggg cgccgccaga ggcaatctcg tgcaggttag acaggtcgta cttgtcgatc
      781 agggtgctct tagcgaagaa gctgaacagg gttggcacca gcagggcaga ctgaattttg
      841 tagtcttgca ggctgcgcag gaacagctcc tcctcgaagc ggtacatcag caccacccga
      901 aagccgcaaa tcaggtagcc cagggtggtg aacatgccga agccgtggtg gaatggcacc
      961 acgctcagaa tagcggtgtc ggggatgatc tggttgccga aaatagg
//
