LOCUS       sequence-149483-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast drug counter-selectable selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pFA6a-FTKnatAX
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(37..70)
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gctgcaggtc gacggatccc cgggttaatt aagatctacc gttcgtatag catacattat
       61 acgaagttat cctagtcata tgcctacaac aactaagaaa atggctatca tgcggaagct
      121 ggtgagaaga acagcatcgg gacaagggaa ggaagaacaa agacaaagaa aacaaaagaa
      181 agcaattgaa aacaaaacaa aacaattttc attccttctc ttatcattcc ttttcttttc
      241 ttttctctca ttcaacgcac tccatcgtat ccgtattcct cttatttttt ctctttctct
      301 atatccattt ctttctctct aggtgtgtcc tctctctctc ttcaatttct ctactccgca
      361 ttccaacgca tccttccccc aacctcccat ttcctcctta cggcccgata gcgatcgtct
      421 ttccctcgct atcactcgct accggcccct cctctgcacc gtaacctcct acgtatttac
      481 catatcataa agttttttcc gacgcttatc gctgaccccc tgtcgccctc ctattggctt
      541 ccggattatc ttcttgtcca taaggtgatc catgcttcct gaagattccc gaaatgtgtc
      601 cactttggcg gggaatcatt ccatccactt ctttctctct cgctttcctc attcggcgct
      661 ccccttccgc gtctcattgg tcttccgctc cgtttttgct ttgccgatgt tacttgggga
      721 gaggtgcgat aatcctttcg caaaaactcg gtttgacgcc tcccatggta taaatagtgg
      781 gtggtggaca ggtgccttcg cttttctttt ctttaagcaa gagaagcttc catggaaact
      841 agtgcgttcg accaggctgc gcgttctcgc ggccatagca accgacgtac ggcgttgcgc
      901 cctcgccggc agcaagaagc cacggaagtc cgcctggagc aga
//
