LOCUS       sequence-149485-         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3a-HA3
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..63
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             67..93
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             100..126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      159..346
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(214..238)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        405..748
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(816..835)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ggtcgacgga tccccgggtt aattaacgct agcatgtacc catacgatgt tcctgactat
       61 gcgggctacc cgtatgacgt gccggactat gcaggagcct atccttatga cgttccagat
      121 tacgctggtt ctagatgagg cgcgccactt ctaaataagc gaatttctta tgatttatga
      181 tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt
      241 aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt
      301 ctcaggtata gtatgaggtc gctcttattg accacacctc taccggcaga tccgctagga
      361 gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga
      421 ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt
      481 acatttagcc catacatccc catgtataat catttgcatc catacatttt gatggccgca
      541 cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc
      601 tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg
      661 atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt
      721 ctcacatcac atccgaacat aaacaaccat gggtaaggaa aagactcacg tttcgaggcc
      781 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
      841 cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cag
//
