LOCUS       sequence-149486-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3a-HA3
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      67..264
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(323..340)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(574..593)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
       61 atcagtactg acaataaaaa gattcttgtt ttcaagaact tgtcatttgt atagtttttt
      121 tatattgtag ttgttctatt ttaatcaaat gttagcgtga tttatatttt ttttcgcctc
      181 gacatcatct gcccagatgc gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt
      241 atgtgaatgc tggtcgctat actgctgtcg attcgatact aacgccgcca tccagtttaa
      301 acgagctcga attctaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      361 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      421 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      481 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      541 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      601 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      661 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      721 aggtcgttcg ctccaagctg
//
