LOCUS       sequence-149487-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3a-Myc13
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..547
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
     terminator      610..797
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(665..689)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 tgcaggtcga cggatccccg ggttaattaa cgctagcgcg attaacggtg aacaaaagct
       61 aatctccgag gaagacttga acggtgaaca aaaattaatc tcagaagaag acttgaacgg
      121 actcgacggt gaacaaaagt tgatttctga agaagatttg aacggtgaac aaaagctaat
      181 ctccgaggaa gacttgaacg gtgaacaaaa attaatctca gaagaagact tgaacggact
      241 cgacggtgaa caaaagttga tttctgaaga agatttgaac ggtgaacaaa agctaatctc
      301 cgaggaagac ttgaacggtg aacaaaaatt aatctcagaa gaagacttga acggactcga
      361 cggtgaacaa aagttgattt ctgaagaaga tttgaacggt gaacaaaagc taatctccga
      421 ggaagacttg aacggtgaac aaaaattaat ctcagaagaa gacttgaacg gactcgacgg
      481 tgaacaaaag ttgatttctg aagaagattt gaacggtgaa caaaagctaa tctccgagga
      541 agacttgaac ggtgaacaaa aattaatcaa tcagtacaag tctagatgag gcgcgccact
      601 tctaaataag cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa
      661 ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg
      721 agtaactctt tcctgtaggt caggttgctt tctcaggtat agtatgaggt cgctcttatt
      781 gaccacacct ctaccggcag atccgctagg agatctgttt agcttgcctc gtccccgccg
      841 ggtcacccgg ccagcgacat ggaggcccag aataccctcc ttgacagtct tgacgtgcgc
      901 agctc
//
