LOCUS       sequence-149489-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3a-FL3
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..66
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             70..117
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     terminator      147..334
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(202..226)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        393..736
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(804..823)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gcaggtcgac ggatccccgg gttaattaac gctagcatgg gtgactacaa ggacgacgat
       61 gacaagggag attacaaaga tgacgacgat aaggactaca aggacgacga tgacaagtct
      121 agatgaggcg cgccacttct aaataagcga atttcttatg atttatgatt tttattatta
      181 aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac
      241 gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagt
      301 atgaggtcgc tcttattgac cacacctcta ccggcagatc cgctaggaga tctgtttagc
      361 ttgcctcgtc cccgccgggt cacccggcca gcgacatgga ggcccagaat accctccttg
      421 acagtcttga cgtgcgcagc tcaggggcat gatgtgactg tcgcccgtac atttagccca
      481 tacatcccca tgtataatca tttgcatcca tacattttga tggccgcacg gcgcgaagca
      541 aaaattacgg ctcctcgctg cagacctgcg agcagggaaa cgctcccctc acagacgcgt
      601 tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa aggttaggat ttgccactga
      661 ggttcttctt tcatatactt ccttttaaaa tcttgctagg atacagttct cacatcacat
      721 ccgaacataa acaaccatgg gtaaggaaaa gactcacgtt tcgaggccgc gattaaattc
      781 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
      841 tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgt
//
