LOCUS       sequence-149491-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3a-PK6
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..73
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             77..118
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             119..160
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             167..208
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             212..253
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             254..295
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     terminator      325..512
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(380..404)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        571..914
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 tcgacggatc cccgggttaa ttaacgctag cggtaagcct attcctaacc ctcttttggg
       61 tctcgattct acaggtggaa aaccaatccc caacccactc ctcggccttg actcaactgg
      121 caaacccata ccaaatcctt tacttggatt agatagtacc tctagcggta agcctattcc
      181 taaccctctt ttgggtctcg attctacagg tggaaaacca atccccaacc cactcctcgg
      241 ccttgactca actggcaaac ccataccaaa tcctttactt ggattagata gtacctctag
      301 atgaggcgcg ccacttctaa ataagcgaat ttcttatgat ttatgatttt tattattaaa
      361 taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga
      421 aaattcttat tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagtat
      481 gaggtcgctc ttattgacca cacctctacc ggcagatccg ctaggagatc tgtttagctt
      541 gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac
      601 agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata
      661 catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa
      721 aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac agacgcgttg
      781 aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg
      841 ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc
      901 gaacataaac aaccatg
//
