LOCUS       sequence-149493-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3a-PK3
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..74
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             78..119
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             120..161
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     terminator      191..378
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(246..270)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        437..780
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(848..867)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gtcgacggat ccccgggtta attaacgcta gcggtaagcc tattcctaac cctcttttgg
       61 gtctcgattc tacaggtgga aaaccaatcc ccaacccact cctcggcctt gactcaactg
      121 gcaaacccat accaaatcct ttacttggat tagatagtac ctctagatga ggcgcgccac
      181 ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa
      241 aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt
      301 gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg tcgctcttat
      361 tgaccacacc tctaccggca gatccgctag gagatctgtt tagcttgcct cgtccccgcc
      421 gggtcacccg gccagcgaca tggaggccca gaataccctc cttgacagtc ttgacgtgcg
      481 cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc cccatgtata
      541 atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt acggctcctc
      601 gctgcagacc tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt gtccccacgc
      661 cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct tctttcatat
      721 acttcctttt aaaatcttgc taggatacag ttctcacatc acatccgaac ataaacaacc
      781 atgggtaagg aaaagactca cgtttcgagg ccgcgattaa attccaacat ggatgctgat
      841 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga
      901 ttgtatggga agc
//
