LOCUS       sequence-149520-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rat Thioredoxin Reductase using an open reading frame in 
            fusion with a mutated E. coli-type SECIS element.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Arner ES, Sarioglu H, Lottspeich F, Holmgren A, Bock A
  TITLE     High-level expression in Escherichia coli of 
            selenocysteine-containing rat thioredoxin reductase utilizing gene 
            fusions with engineered bacterial-type SECIS elements and 
            co-expression with the selA, selB and selC genes.
  JOURNAL   J Mol Biol. 1999 Oct 8;292(5):1003-16.
  PUBMED    10512699
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "1999-10-8"; volume: "292(5)"; pages: "1003-16"
COMMENT     Sequence Label: pET-TRSter
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg
       61 tcgacggccg cataggctaa cgattggtgc agacctgcaa ccgattatta gcctcagcat
      121 ccggactgga ggatgtctcc cccagaacgc ttagtcaccg acagcgttgt aaatatctct
      181 gcacagaccg ggtggatgcc aatggtgctg tccagctgct gcttggtcag cccgcacttg
      241 agtgcggctg caaagccctg cgtcacctct ccagcatttg gacccagtac gtggaagccc
      301 acgacacgtt cgttgtcttt aaggttacag atgacttttg cataacattt gttgttatcc
      361 cgggatggaa ctgtccattc caatggccag aagaaactgt gataaacttc aatattttct
      421 tccccaaatt tctctacagc tttttcttca gagaggccac agcagccata ctccaaagga
      481 gtaaacacag tcgttgggac attgtcatag tcacatttga cagtggagcc gccatacagc
      541 ctctgagcca gcaatctccc cgcctggatg gccacggggg tcagctccag cttcccctcc
      601 agaatgtcac caatggcgta gatgtaaggc acattggtct gctcctcatc cgtgacaggt
      661 atcttcccgg tcttttcatt gatcttcacg cccacggtct ctaagccaat agttcttgta
      721 caagaatctc ttcctactgc aagcaacact gtgttaaatt cgtcttctat ggtctcctca
      781 ctgtttgtgg atttagcggt caccttgagt cggcctggtg tccctgcttc aatctgttca
      841 attt
//
