LOCUS       sequence-149531-        1849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET biosensor. Visualization of FAK activity at plasma membrane 
            outside lipid rafts in live cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Seong J, Ouyang M, Kim T, Sun J, Wen PC, Lu S, Zhuo Y, Llewellyn NM,
            Schlaepfer DD, Guan JL, Chien S, Wang Y
  TITLE     Detection of focal adhesion kinase activation at membrane 
            microdomains by fluorescence resonance energy transfer.
  JOURNAL   Nat Commun. 2011 Jul 26;2:406. doi: 10.1038/ncomms1414.
  PUBMED    21792185
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2011-07-26"; volume: "2:406. doi"; pages: " 
            10.1038/ncomms1414"
COMMENT     Sequence Label: KRas FAK biosensor
FEATURES             Location/Qualifiers
     source          1..1849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(8..10,11..13,14..526)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(53..74)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(314..333)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     661..682
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1088..1798
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     LCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLEF
                     LTAAGITEGMNELYK"
ORIGIN
        1 ggatcccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
       61 gctggacggc gacgtaaacg gccacaggtt cagcgtgtcc ggcgagggcg agggcgatgc
      121 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      181 gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca
      241 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgtac
      301 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      361 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      421 ggggcacaag ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca
      481 gaagaacggc atcaaggccc acttcaagat ccgccacaac atcgaggacg gcagcgtgca
      541 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      601 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
      661 catggtcctg ctggagttcg tgaccgccgc ccgcatgcat tggtattttg ggaagatcac
      721 tcgtcgggag tccgagcggc tgctgctcaa ccccgaaaac ccccggggaa ccttcttggt
      781 ccgggagagc gagacgacaa aaggtgccta ttgcctctcc gtttctgact ttgacaacgc
      841 caaggggctc aatgtgaagc actacaagat ccgcaagctg gacagcggcg gcttctacat
      901 cacctcacgc acacagttca gcagcctgca gcagctggtg gcctactact ccaaacatgc
      961 tgatggcttg tgccaccgcc tgactaacgt ctgtgggtct acatctggat ctgggaagcc
     1021 gggttctggt gagggttctg agaccgacga ctacgctgag atcatcgacg aggaggagct
     1081 cgagctctct aaaggtgaag aattattcac tggtgttgtc ccaattttgg ttgaattaga
     1141 tggtgatgtt aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg atgctacgta
     1201 cggtaaattg accttaaaat tactctgtac tactggtaaa ttgccagttc catggccaac
     1261 cttagtcact actttaggtt atggtgttca atgttttgct agatacccag atcatatgaa
     1321 acaacatgac tttttcaagt ctgccatgcc agaaggttat gttcaagaaa gaactatttt
     1381 tttcaaagat gacggtaact acaagaccag agctgaagtc aagtttgaag gtgatacctt
     1441 agttaataga atcgaattaa aaggtattga ttttaaagaa gatggtaaca ttttaggtca
     1501 caaattggaa tacaactata actctcacaa tgtttacatc actgctgaca aacaaaagaa
     1561 tggtatcaaa gctaacttca aaattagaca caacattgaa gatggtggtg ttcaattagc
     1621 tgaccattat caacaaaata ctccaattgg tgatggtcca gtcttgttac cagacaacca
     1681 ttacttatcc tatcaatctg ccttattcaa agatccaaac gaaaagagag accacatggt
     1741 cttgttagaa tttttgactg ctgctggtat taccgagggt atgaatgaat tgtacaaaaa
     1801 gaagaagaag aagaagagca agaccaagtg cgtgatcatg taagaattc
//
