LOCUS       sequence-149535-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To purify GST-tagged  recombinant protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Kim JH, Park KW, Lee EW, Jang WS, Seo J, Shin S, Hwang KA, Song J
  TITLE     Suppression of PPARgamma through MKRN1-mediated ubiquitination and 
            degradation prevents adipocyte differentiation.
  JOURNAL   Cell Death Differ. 2014 Apr;21(4):594-603. doi: 
            10.1038/cdd.2013.181. Epub 2013 Dec 13.
  PUBMED    24336050
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cdd.2013.181"; journalName: "Cell Death Differ"; date: 
            "2014-04"; volume: "21"; issue: "4"; pages: "594-603"
COMMENT     Sequence Label: PGEX 4T-1-PPARgamma2
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..22
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ggatctggtt ccgcgtggat ccccggaatt cccgggtcga ctcgagatgg gtgaaactct
       61 gggagattct cctgttgacc cagagcatgg tgccttcgct gatgcactgc ctatgagcac
      121 ttcacaagaa attaccatgg ttgacacaga gatgccattc tggcccacca acttcggaat
      181 cagctctgtg gacctctccg tgatggaaga ccactcgcat tcctttgaca tcaagccctt
      241 taccacagtt gatttctcca gcatttctgc tccacactat gaagacattc cattcacaag
      301 agctgaccca atggttgctg attacaaata tgacctgaag ctccaagaat accaaagtgc
      361 gatcaaagta gaacctgcat ctccacctta ttattctgaa aagacccagc tctacaacag
      421 gcctcatgaa gaaccttcta actccctcat ggccattgag tgccgagtct gtggggataa
      481 agcatcaggc ttccactatg gagttcatgc ttgtgaagga tgcaagggtt ttttccgaag
      541 aaccatccga ttgaagctta tttatgatag gtgtgatctt aactgccgga tccacaaaaa
      601 aagtagaaat aaatgtcagt actgtcggtt tcagaagtgc cttgctgtgg ggatgtctca
      661 caatgccatc aggtttgggc ggatgccaca ggccgagaag gagaagctgt tggcggagat
      721 ctccagtgat atcgaccagc tgaacccaga gtctgctgat ctgcgagccc tggcaaagca
      781 tttgtatgac tcatacataa agtccttccc gctgaccaaa gccaaggcga gggcgatct
//
