LOCUS       sequence-149539-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3c-PK12
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..80
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             84..125
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             126..167
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             174..215
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             219..260
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             261..302
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             309..350
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             354..395
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             396..437
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             444..485
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             489..530
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             531..572
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     terminator      605..792
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(660..684)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 ctgcaggtcg acggatcccc gggttaatta acgctagcgg taagcctatt cctaaccctc
       61 ttttgggtct cgattctaca ggtggaaaac caatccccaa cccactcctc ggccttgact
      121 caactggcaa acccatacca aatcctttac ttggattaga tagtacctct agcggtaagc
      181 ctattcctaa ccctcttttg ggtctcgatt ctacaggtgg aaaaccaatc cccaacccac
      241 tcctcggcct tgactcaact ggcaaaccca taccaaatcc tttacttgga ttagatagta
      301 cctctagcgg taagcctatt cctaaccctc ttttgggtct cgattctaca ggtggaaaac
      361 caatccccaa cccactcctc ggccttgact caactggcaa acccatacca aatcctttac
      421 ttggattaga tagtacctct agcggtaagc ctattcctaa ccctcttttg ggtctcgatt
      481 ctacaggtgg aaaaccaatc cccaacccac tcctcggcct tgactcaact ggcaaaccca
      541 taccaaatcc tttacttgga ttagatagta cctctagaga ttgaggcgcg ccacttctaa
      601 ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag
      661 tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa
      721 ctctttcctg taggtcaggt tgctttctca ggtatagtat gaggtcgctc ttattgacca
      781 cacctctacc ggcagatccg ctaggagatc tgtttagctt gcctcgtccc cgccgggtca
      841 cccggccagc gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc
      901 aggggcatga tgtgactgtc gcccgta
//
