LOCUS       sequence-149540-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress PPARgamma2 2KR in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Kim JH, Park KW, Lee EW, Jang WS, Seo J, Shin S, Hwang KA, Song J
  TITLE     Suppression of PPARgamma through MKRN1-mediated ubiquitination and 
            degradation prevents adipocyte differentiation.
  JOURNAL   Cell Death Differ. 2014 Apr;21(4):594-603. doi: 
            10.1038/cdd.2013.181. Epub 2013 Dec 13.
  PUBMED    24336050
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cdd.2013.181"; journalName: "Cell Death Differ"; date: 
            "2014-04"; volume: "21"; issue: "4"; pages: "594-603"
COMMENT     Sequence Label: pcDNA3.1-PPARgamma2 2KR
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgtttaaact taagcttggt accgagctcg
      121 gatccactag tccagtgtgg tggaattctg cagatatcca gcacagtggc ggccgctcga
      181 gatgggtgaa actctgggag attctcctgt tgacccagag catggtgcct tcgctgatgc
      241 actgcctatg agcacttcac aagaaattac catggttgac acagagatgc cattctggcc
      301 caccaacttc ggaatcagct ctgtggacct ctccgtgatg gaagaccact cgcattcctt
      361 tgacatcaag ccctttacca cagttgattt ctccagcatt tctgctccac actatgaaga
      421 cattccattc acaagagctg acccaatggt tgctgattac aaatatgacc tgaagctcca
      481 agaataccaa agtgcgatca aagtagaacc tgcatctcca ccttattatt ctgaaaagac
      541 ccagctctac aacaggcctc atgaagaacc ttctaactcc ctcatggcca ttgagtgccg
      601 agtctgtggg gataaagcat caggcttcca ctatggagtt catgcttgtg aaggatgcaa
      661 gggttttttc cgaagaacca tccgattgaa gcttatttat gataggtgtg atcttaactg
      721 ccggatccac aggaggagta gaaataaatg tcagtactgt cggtttcaga agtgccttgc
      781 tgtggggatg tctcacaatg ccatcaggtt tgggcggatg ccacaggccg agaaggagaa
      841 gctgttggcg gagatctcca gtgatatcga ccagctgaac ccagagtctg ctgatctgcg
      901 agccctggca aagcatttgt atgactcata cataa
//
