LOCUS       sequence-149542-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3c-PK12
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(33..230)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(236..808)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
ORIGIN
        1 actggatggc ggcgttagta tcgaatcgac agcagtatag cgaccagcat tcacatacga
       61 ttgacgcatg atattacttt ctgcgcactt aacttcgcat ctgggcagat gatgtcgagg
      121 cgaaaaaaaa tataaatcac gctaacattt gattaaaata gaacaactac aatataaaaa
      181 aactatacaa atgacaagtt cttgaaaaca agaatctttt tattgtcagt actgattagg
      241 ggcagggcat gctcatgtag agcgcctgct cgccgtccga ggcggtgccg tcgtacaggg
      301 cggtgtccag gccgcagagg gtgaacccca tccgccggta cgcgtggatc gccggtgcgt
      361 tgacgttggt gacctccagc cagaggtgcc cggcgccccg ctcgcgggcg aactccgtcg
      421 cgagccccat caacgcgcgc ccgaccccgt gcccccggtg ctccggggcg acctcgatgt
      481 cctcgacggt cagccggcgg ttccagccgg agtacgagac gaccacgaag cccgccaggt
      541 cgccgtcgtc cccgtacgcg acgaacgtcc gggagtccgg gtcgccgtcc tccccgtcgt
      601 ccgattcgtc gtccgattcg tcgtcgggga acaccttggt caggggcggg tccaccggca
      661 cctcccgcag ggtgaagccg tccccggtgg cggtgacgcg gaagacggtg tcggtggtga
      721 aggacccatc cagtgcctcg atggcctcgg cgtcccccgg gacactggtg cggtaccggt
      781 aagccgtgtc gtcaagagtg gtacccatgg ttgtttatgt tcggatgtga tgtgagaact
      841 gtatcctagc aaga
//
