LOCUS       sequence-149547-        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3c-HA3
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..74
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             78..104
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             111..137
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      170..357
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(225..249)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        416..759
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 cgtacgctgc aggtcgacgg atccccgggt taattaacgc tagcatgtac ccatacgatg
       61 ttcctgacta tgcgggctac ccgtatgacg tgccggacta tgcaggagcc tatccttatg
      121 acgttccaga ttacgctggt tctagatgag gcgcgccact tctaaataag cgaatttctt
      181 atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat acaaatttta
      241 aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt tcctgtaggt
      301 caggttgctt tctcaggtat agtatgaggt cgctcttatt gaccacacct ctaccggcag
      361 atccgctagg agatctgttt agcttgcctc gtccccgccg ggtcacccgg ccagcgacat
      421 ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga
      481 ctgtcgcccg tatatttagc ccacacatcc ccatgtataa tcatttgcat ccatacattt
      541 tgatggctgc acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg
      601 aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat
      661 aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct
      721 aggatacagt tctcacatca catccgaaca taaacaacca tgggtaccac tcttgacgac
      781 acggcttacc ggtaccgcac cagtgtcccg ggggacgccg aggccatcga ggcactggat
      841 gggtccttca ccaccgacac cgtcttccgc gtcaccgcca ccggggacgg cttcaccctg
      901 cgggaggtgc cggtggaccc gcccctgacc aaggtgttcc ccgacgacga atcggacgac
      961 gaatcggacg acggggagga cggcgacccg gactcccgga cgttcgtcgc gtacggggac
     1021 gacggc
//
