LOCUS       sequence-149548-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress PPARgamma2 in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Kim JH, Park KW, Lee EW, Jang WS, Seo J, Shin S, Hwang KA, Song J
  TITLE     Suppression of PPARgamma through MKRN1-mediated ubiquitination and 
            degradation prevents adipocyte differentiation.
  JOURNAL   Cell Death Differ. 2014 Apr;21(4):594-603. doi: 
            10.1038/cdd.2013.181. Epub 2013 Dec 13.
  PUBMED    24336050
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cdd.2013.181"; journalName: "Cell Death Differ"; date: 
            "2014-04"; volume: "21"; issue: "4"; pages: "594-603"
COMMENT     Sequence Label: PCS4 3XFLAG-PPARgamma2
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        53..71
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     53..70
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             147..170
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             174..197
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             201..224
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcatgctcgt ttagggaccg ccattctgcc tggggacgtc ggagcaagct tgatttaggt
       61 gacactatag aatacaagct acttgttctt tttgcaggat cccatcgatt gtacagatat
      121 ctggcgcgcc taccatgggg cccggcgact acaaggatga cgatgacaag ggcgactaca
      181 aggatgacga tgacaagggc gactacaagg atgacgatga caagggccgg cctaagatct
      241 ggaattcaag gcctctcgag atgggtgaaa ctctgggaga ttctcctgtt gacccagagc
      301 atggtgcctt cgctgatgca ctgcctatga gcacttcaca agaaattacc atggttgaca
      361 cagagatgcc attctggccc accaacttcg gaatcagctc tgtggacctc tccgtgatgg
      421 aagaccactc gcattccttt gacatcaagc cctttaccac agttgatttc tccagcattt
      481 ctgctccaca ctatgaagac attccattca caagagctga cccaatggtt gctgattaca
      541 aatatgacct gaagctccaa gaataccaaa gtgcgatcaa agtagaacct gcatctccac
      601 cttattattc tgaaaagacc cagctctaca acaggcctca tgaagaacct tctaactccc
      661 tcatggccat tgagtgccga gtctgtgggg ataaagcatc aggcttccac tatggagttc
      721 atgcttgtga aggatgcaag ggttttttcc gaagaaccat ccgattgaag cttatttatg
      781 ataggtgtga tcttaactgc cggatccaca aaaaaagtag aaataaatgt cagtactgtc
      841 ggtttcagaa gtgccttgct gtggggatgt ctcacaatgc catcaggttt gggcggatgc
      901 cacaggccga gaag
//
