LOCUS       sequence-149559-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast drug selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pFA6a-TKnatCX
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(16..213)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(219..791)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
ORIGIN
        1 gtatcgaatc gacagcagta tagcgaccag cattcacata cgattgacgc atgatattac
       61 tttctgcgca cttaacttcg catctgggca gatgatgtcg aggcgaaaaa aaatataaat
      121 cacgctaaca tttgattaaa atagaacaac tacaatataa aaaaactata caaatgacaa
      181 gttcttgaaa acaagaatct ttttattgtc agtactgatt aggggcaggg catgctcatg
      241 tagagcgcct gctcgccgtc cgaggcggtg ccgtcgtaca gggcggtgtc caggccgcag
      301 agggtgaacc ccatccgccg gtacgcgtgg atcgccggtg cgttgacgtt ggtgacctcc
      361 agccagaggt gcccggcgcc ccgctcgcgg gcgaactccg tcgcgagccc catcaacgcg
      421 cgcccgaccc cgtgcccccg gtgctccggg gcgacctcga tgtcctcgac ggtcagccgg
      481 cggttccagc cggagtacga gacgaccacg aagcccgcca ggtcgccgtc gtccccgtac
      541 gcgacgaacg tccgggagtc cgggtcgccg tcctccccgt cgtccgattc gtcgtccgat
      601 tcgtcgtcgg ggaacacctt ggtcaggggc gggtccaccg gcacctcccg cagggtgaag
      661 ccgtccccgg tggcggtgac gcggaagacg gtgtcggtgg tgaaggaccc atccagtgcc
      721 tcgatggcct cggcgtcccc cgggacactg gtgcggtacc ggtaagccgt gtcgtcaaga
      781 gtggtaccca tggcccgggc a
//
